电针对戊四唑点燃型癫痫大鼠脑电及海马神经元内Ca~(2+)含量的影响

目的:探讨电针抗癫痫的可能机制。方法:SD大鼠20只随机分为正常组、模型组、尼莫地平组、电针组。除正常组外,其它3组均制作戊四唑慢性点燃癫痫模型。电针组电针"百会"大椎",尼莫地平组腹腔注射尼莫地平。记录各组大鼠治疗后痫样波发作潜伏期及痫波密度,使用激光共聚焦显微镜观察大鼠海马神经元内Ca2+的荧光强度。结果:电针组脑电痫样波发作潜伏期较模型组明显延长(P<0.05),痫波密度与模型组比较明显变小(P<0.05)。模型组大鼠海马神经元Ca2+荧光强度较正常组明显升高(P<0.01),电针组Ca2+荧光强度较模型组明显降低(P<0.05),尼莫地平组Ca2+荧光强度较模型组明显降低(P<0.01)。结论:电针可调节戊四唑点燃型癫痫大鼠脑电及海马内Ca2+含量,电针的抗癫痫作用可能与之有关。

Effect of Electroacupuncture on Epileptic EEG and Intracellular Ca~(2+) Content in the Hippocampus in Epilepsy Rats

Objective To study the underlying mechanism of electroacupuncture (EA) in relieving epilepsy in pentyle-netetrazole (PTZ)-induced epilepsy rats. Methods Twenty SD rats were randomly divided into normal control, model, EA, Ni-modipine groups, with 5 cases in each. Epilepsy model was established by intraperitoneal injection of PTZ (32. 0 mg/kg), once daily for 28 days. EA (100 Hz, 0. 6 mA) was applied to "Baihui"(GV 20) and "Dazhui"(GV 14), once daily for 7 days. For Ni-modipine group, the rats were given with nimodipine (0. 25 mg/kg, i.p.), once daily for 7 days. Electroencephalogram (EEG) was recorded and the fluorescence intensity of Ca~(2+) of the hippocampus tissue sections was detected by laser scanning confocal microscope (LSCM) after incubation in artificial cerebrospinal fluid containing Flou-3/AM (10 μmol/L) and pluronic F-127 (5 μl). Results Compared with model group, the latencies of epileptic EEG seizure prolonged obviously (P<0. 05), and epileptic EEG seizure frequencies decreased significantly (P<0. 05) in EA and Nimodipine groups. The fluorescence intensity of intracellular Ca~(2+) in hippocampus tissue in model group was obviously higher than that in control group (P<0. 01). In comparison to model group, Ca2+ levels in EA and Nimodipine groups lowered considerably (P<0. 05, P<0. 01). No significant differences were found between EA and Nimodipine groups in the aforementioned 3 indexes (P>0. 05). Conclusion EA has an obvious anti-epileptic effect, which may be closely related to its effect in downregulating the increased hippocampal Ca~(2+) level in PTZ-kindled epilepsy rats.