电针不同穴组防治脑心综合征作用机制的研究

目的:筛选出针刺防治脑心综合征的有效穴组,并初步探讨针刺防治脑心综合征的作用机制。方法:Wistar大鼠随机分为正常组(n=8)、伪手术组(n=8)、模型组(n=12)、水沟-风府组(n=12)、内关-心俞组(n=12)。采用胶原酶加肝素联合注射建立脑出血动物模型。水沟-风府组选取"水沟""风府"予以治疗,内关-心俞组选取"内关""心俞"予以治疗,每次电针20 min,每天1次,共3次。采用心率变异性分析系统检测大鼠心率变异性,采用高效液相色谱法测定大鼠脑组织兴奋性氨基酸(EAA)含量。结果:与伪手术组比较,模型组R-R间期(RRI)延长,R-R间期总变异(TV)增大,低频成分/高频成分(LF/HF)减小(P<0.01);两针刺组与模型组比较,RRI缩短,TV减小,LF/HF增大(P<0.01);针刺两组之间比较,差异无统计学意义(P>0.05)。与伪手术组比较,模型组谷氨酸(Glu)、天冬氨酸(Asp)含量显著升高(P<0.05,P<0.01);两针刺组大鼠脑组织内Glu、Asp含量均低于模型组(P<0.05);针刺两组之间比较,脑组织Glu、Asp含量的差异无统计学意义(P>0.05)。结论:电针"水沟""风府"或"内关""心俞"均可防治脑心综合征,且两穴组可相互替代。交感神经系统兴奋性降低及脑内EAA的水平升高可能参与脑心综合征的发生发展;电针降低脑出血模型大鼠脑内EAA的水平可能是其防治脑心综合征的作用机制之一。

Study on the Mechanism of Electroacupuncture of Different Acupoint Groups in the Treatment of Cerebrocardiac Syndrome in Rats

Objective To compare the effects of electroacupuncture (EA) of different acupoint groups on heart rate variability (HRV) and cerebral glutamate (Glu) and aspartic acid (Asp) contents in cerebrocardiac syndrome (CCS) rats. Methods A total of 52 Wistar rats were randomized into normal (n = 8), sham-operation (sham, n = 8), model (n = 12), Shuigou-Fengfu (GV 26-GV 16, n = 12), and Neiguan-Xinshu (PC 6-BL 15, n = 12) groups. CCS model was established by intracerebral (Caudate nucleus) injection of clostridiopeptidase (1 U/μl) plus heparin (7 U/μl). EA (2 Hz/100 Hz, 1-3 mA) was applied to GV 26-GV 16 and PC 6-BL 15 for 20 min, once daily for 3 days. Cerebral Glu and Asp contents were detected by high perfor-mance liquid chromatography, and HRV was analyzed by SMUP Software. Results In comparison with normal and sham groups, the R-R interval (RRI) of electrocardiogram and total variability (TV) of HRV, cerebral Glu and Asp contents in model group increased significantly (P<0. 01, P<0. 05), and the ratio of low frequency/high frequency (LF/HF) decreased evidently (P<0. 01). Compared with model group, RRI and TV values, and cerebral Glu and Asp contents of EA of GV 26-GV 16 and PC 6-BL 15 groups reduced markedly (P<0. 01, P<0. 05), LF/HF of the later two groups increased evidently (P<0. 01). No significant differences were found between GV 26-GV 16 and PC 6-BL 15 groups in RRI, TV and LF/HF of HRV, and cerebral Glu and Asp contents (P>0. 05). Conclusion EA of both GV 26-GV 16 and PC 6-BL 15 can effectively improve CCS in rats, which is closely associated with their effects in lowering cerebral Glu and Asp levels and in suppressing the excitability of sympathetic nerve.