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电针对膝骨性关节炎家兔模型白介素-1β和肿瘤坏死因子-α的影响
引用本文:黄剑,卓廉士,王永渝,彭支莲,黄英如,汪莹,杨里.电针对膝骨性关节炎家兔模型白介素-1β和肿瘤坏死因子-α的影响[J].针刺研究,2007,32(2):115-118.
作者姓名:黄剑  卓廉士  王永渝  彭支莲  黄英如  汪莹  杨里
作者单位:1. 重庆医科大学中医药学院,重庆,400050
2. 重庆市九龙坡区第一中医院,重庆,400080
摘    要:目的:观察电针对膝骨性关节炎家兔模型关节冲洗液中细胞因子白介素-1β(IL-1β)和肿瘤坏死因子-α(TNF-α)的影响。方法:将新西兰大白兔40只随机分为空白对照组(A组)、模型对照组(B组)、按摩组(C组)、电针治疗组(D组)。除A组外,将其余3组大白兔左膝关节伸直位石膏固定7周制成骨性关节炎动物模型。造模成功后,A组和B组正常饲养,不处理;C组给予推拿按摩加功能训练;D组取左后肢“内膝眼、外膝眼、阳陵泉、血海、足三里和梁丘”6个穴位电针治疗,电压6V,疏密波形,频率1.6-2Hz,输出强度1-3mA,每天1次,每次电针30min,连续治疗21d。处理前、后分别取各组家兔关节冲洗液,采用放射免疫分析法,测定其中IL-1β和TNF-α的含量。结果:经治疗后,C、D组关节液中IL-1β和TNF-α含量与B组比较明显降低,有显著性差异(P<0.05);C、D组治疗前后自身比较亦有显著性差异(P<0.05)。结论:电针治疗能降低膝骨性关节炎家兔关节液中细胞因子IL-1β和TNF-α的含量,从而发挥对膝骨性关节炎的防治作用。

关 键 词:膝骨性关节炎  电针  细胞因子
文章编号:1000-0607(2007)02-0115-04
收稿时间:10 9 2006 12:00AM
修稿时间:12 18 2006 12:00AM

Effects of Electroacupuncture on Synovia IL-1β and TNF-α Contents in the Rabbit with Knee Osteoarthritis
HUANG Jian,ZHUO Lian-shi,WANG Yong-yu,PENG Zhi-lian,HUANG Ying-ru,WANG Ying,YANG Li.Effects of Electroacupuncture on Synovia IL-1β and TNF-α Contents in the Rabbit with Knee Osteoarthritis[J].Acupuncture Research,2007,32(2):115-118.
Authors:HUANG Jian  ZHUO Lian-shi  WANG Yong-yu  PENG Zhi-lian  HUANG Ying-ru  WANG Ying  YANG Li
Institution:College of Chinese Medicine, Chongqing Medical University, Chongqing 400050, China; 1 The First Chinese Medical Hospital of Jiulongpo Zone, Chongqing 400080
Abstract:OBJECTIVE: To observe the effect of electroacupuncture (EA) on synovia IL-1beta and TNF-alpha contents in rabbits with knee osteoarthritis (KOA). METHODS: A total of 40 New Zealand rabbits were randomly divided into control, model, massage and EA groups. KOA model was established by gypsum fixing method. EA (2 Hz, 1-3 mA) was applied to left "Xiyan" (EX-LE 5), "Yanglingquan" (GB 34), "Xuehai" (SP 10), "Zusanli" (ST 36) and "Liangqiu" (ST 34) for 30 min, once daily and continuously for 21 days. For massage group, the affected knee joint was pressed, kneaded, stretched and rotated repeatedly for 15 min every time, followed by forced running about 100 m. The intra-joint synovia was collected (0.4-0.6 mL) for detecting the contents of IL-1beta and TNF-alpha with radioimmunoassay. RESULTS: No IL-1beta and TNF-alpha were detected in the synovia in control group, while in the other 3 groups, synovia IL-1beta and TNF-alpha levels increased significantly. Before treatment, no significant differences were found among model, EA and massage groups in the levels of IL-1beta and TNF-alpha (P > 0.05), while after the treatment the two indexes both decreased considerably (P < 0.05). Compared with model group, the two indexes were remarkably lower (P < 0.05), but no significant differences were found between EA and massage groups in synovia IL-1beta and TNF-alpha contents (P > 0.05). CONCLUSION: Both EA and massage can effectively suppress the release of synovia IL-1beta and TNF-alpha in KOA of rabbits, which may contribute to the effect of acupuncture in the treatment of osteoarthritis.
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