电针“大椎”“命门”对佐剂性关节炎大鼠应激相关因子影响的实验研究

目的:通过观察电针“大椎”“命门”穴后下丘脑CRH、血浆ACTH、血清Cort、TNF-α含量的关系,分析电针不同穴位对应激相关因子的影响。方法:以佐剂性关节炎大鼠(AA)作为研究对象,随机分正常组、模型组、电针“大椎”组、电针“命门”组。观察电针对关节炎症局部及下丘脑CRH、血浆ACTH、血清Cort、TNF-α含量的影响。结果:“大椎”组、“命门”组经治疗右后足足爪肿胀率与模型对照组比较极明显降低(P<0.01),“大椎”组下丘脑CRH含量、血清Cort、TNF-α含量,“命门”组血清Cort含量与模型对照组比较明显降低(P<0.05)。结论:电针具有一定的抗炎作用,并且这种作用可能是通过对CRH、Cort等应激相关因子的调节,减轻疾病应激程度而实现的。

Influence of Electroacupuncture of "Dazhui" (GV 14) and "Mingmen" (GV 4) on Stress-related Factors in Adjuvant Arthritis Rats

Objective: To study the effect of electroacupuncture(EA) of "Dazhui"(GV 14) and "Mingmen"(GV 4) on hypothalamic corticotrophin releasing hormone(CRH),plasma adrenocorticotropic hormone(ACTH),serum cortisol(Cort) and tumor necrosis factor(TNF)-α contents in adjuvant arthritis rats.Methods: Thirty-six male Wistar rats were evenly randomized into normal control,model,EA-GV14 and EA-GV4 groups.Arthritis model was established by subcutaneous injection of Freund's complete adjuvant(FCA,(0.1 mL)) into the right hind-paw.EA((15 Hz),continuous pulses,(1 mA)) was applied to "Dazhui"(GV 14) and "Mingmen"(GV 4) for(20 min),once every other day and continuously for 8 times.After the rats were killed,hypothalamic tissue and blood sample were taken for detecting hypothalamic CRH,plasma ACTH,serum Cort and TNF-α contents with radioimmunoassay.Results: Compared with normal control group,the swelling rate of the right hindpaw(24 h) after injection of FCA and 16 days after EA increased significantly(P<0.01);while in comparison with model group,the swelling rates of EA-GV14 and EA-GV4 groups were markedly lower(P<0.01).Compared with normal control group,hypothalamic CRH,plasma ACTH,serum Cort and TNF-α contents of model group increased significantly(P<0.05);while in comparison with model group,hypothalamic CRH serum Cort and TNF-α contents in EA-GV14 group,and serum Cort level in EA-GV4 group decreased significantly(P<0.05).No significant differences were found between EA-GV14 and EA-GV4 groups(P>0.05) in these 4 indexes.Conclusion: EA has a marked anti-inflammatory effect in adjuvant arthritis rats and can relieve arthritis stress induced increase of hypothalamic CRH,plasma ACTH,serum Cort and TNF-α levels.