头针、体针和针药结合治疗对全脑缺血大鼠钙调素活性即刻影响的比较研究

目的 :观察针刺头穴和体穴及结合药物治疗对全脑缺血大鼠活性钙调素含量的影响。方法 :采用大鼠全脑缺血模型 ,分别针刺“水沟”、“百会”穴与针刺“商阳”、“中冲”穴 ,另外分别口饲维脑路通后针刺上述头穴、体穴 ,以测定缺血脑组织活性钙调素 (CaM )的含量。结果 :大鼠全脑缺血后 ,脑组织CaM与正常对照组、假手术组比明显升高 (P <0 0 0 1 ,0 0 1 ,0 0 5) ,治疗组与模型组比明显下降 (P <0 0 0 1 ,0 0 1 ) ,头穴单纯针刺组与体穴单纯针刺组比较及头穴针药结合组与体穴针药结合组比均无显著性差异 (P >0 0 5) ,头穴单纯针刺组与针药结合组比有显著性差异 (P <0 0 5) ,体穴单纯针刺组与针药结合组比仅有降低的趋势 ,但无显著性差异 (P >0 0 5)。结论 :无论针刺头穴还是体穴均可明显降低缺血区脑组织活性钙调素的含量 ,而且头穴针药结合治疗后 ,其作用明显优于单纯针刺 ,说明针刺头穴合并药物治疗脑缺血时 ,针药相引 ,直达病所。

Comparison of Effects of Scalp-acupuncture, Body-acupuncture and Combined Application of Acupuncture and Medicine on Calmodulin Activity in Cerebral Ischemia Rats

Objective: To observe the effect of scalp acupuncture, body acupuncture and joint administration of acupuncture and medicine on cerebral ischemia induced changes of calmodulin (CaM) activity. Methods: 63 Wistar rats were randomly and evenly divided into control, sham operation, model, scalp acupuncture (SA), body acupuncture (BA), SA+medicine and BA+medicine groups. Cerebral ischemia reperfusion (IR) model was established by occlusion of the bilateral vertebral arteries and common carotid artery. Scalp points “Shuigou"(GV 26) and “Baihui"(GV 20), and body acupoints “Shangyang"(LI 1) and “Zhongchong"(PC 9) were punctured and stimulated electrically (20 Hz, 1 mA, continuous waves) for 20 min. In acupuncture+medication group, intra gastric perfusion of Venoruton (0.45 g/kg) was given to the rats. CaM concentration in the brain was determined with radioimmunoassay. Results: In comparison with control and sham operation groups, CaM content of model group increased considerably ( P <0.001). Compared with model group, CaM contents of SA, BA, SA+medicine and BA+medicine groups all decreased significantly ( P <0.01～0.001). In comparison with SA group, CaM contents of both SA+medicine group and BA+medicine group were significantly lower ( P <0.05), but no significant differences were found between control group and sham operation group, among BA, SA+medicine and BA+medicine groups, and between SA and BA groups in CaM contents ( P >0.05). Conclusion: Both scalp acupuncture and body acupuncture and joint administration of acupuncture and medicine can significantly lower CaM content of the brain tissue in cerebral ischemia rats, and the effect of SA+medicine is apparently superior to that of simple acupuncture.