赞育丹对帕罗西汀诱导大鼠性功能障碍的治疗作用及机制※

目的 探讨赞育丹防治帕罗西汀诱导大鼠性功能障碍的效果和机制。方法 将50只性功能正常的雄性Wistar大鼠随机分为5组，每组10只。正常组、模型组分别给予等体积生理盐水、帕罗西汀灌胃，赞育丹低、中、高剂量组分别给予相应剂量（低、中、高剂量）的赞育丹联合帕罗西汀灌胃。连续灌胃4 w后观察各组大鼠性行为参数，并检测血清睾酮、阴茎鸟苷单磷酸（cGMP）、一氧化氮（NO）水平及eNOS、PDE5含量。结果 与模型组相比，赞育丹各给药组的骑跨潜伏期、插入潜伏期及射精潜伏期均剂量依赖性地降低（P<0.05），骑跨次数、血清睾酮和阴茎NO、cGMP水平、eNOS含量均随赞育丹剂量的升高而增加（P<0.05）；赞育丹中、高剂量组的插入次数增加，大鼠阴茎PDE5含量降低（P<0.05）。结论 赞育丹可通过提高血清睾酮，提高阴茎eNOS、NO、cGMP水平，抑制PDE5来缓解帕罗西汀引起的性功能障碍。

Study on the effect of Zan-Yu-Dan on paroxetine-induced sexual dysfunction in rats and the activation of NO/ cGMP pathway

Objective: This study aimed to explore the effects and mechanism of Zan-Yu-Dan (ZYD) on the paroxetine-induced sexual dysfunction in rats. Methods: Total 50 male Wistar rats were divided into five groups, ten rats in each group. The rats in control group were treated with distilled water by intragastric administration; the rats in the model group were treated with 10 mg/kg paroxetine; the rats in the ZYD groups were administered with 10 mg/kg paroxetine and indicated doses of ZYD, respectively. After treatment for 4 weeks, sexual behavior parameters, testosterone levels, NO, cGMP, eNOS and PDE5 levels among all the groups were measured and compared. Results: Compared with model group, the mount latency (ML), intromission latency (IL) and ejaculatory latency (EL) in ZYD groups were significantly decreased with dose-dependent manner (P<0.05). The mount frequency (MF) in ZYD groups were significantly increased along with the dose-dependent manner (P<0.05). The intromission frequency (IF) in high and middle-dose ZYD group was increased significantly (P<0.05). After the treatment, serum testosterone, penile NO and cGMP levels in ZYD groups were increased significantly with the dose-dependent manner (P<0.05). In the model group, the expression level of eNOS was decreased while that of PDE5 was increased, while ZYD significantly increased the expression level of eNOS and decreased PDE5. Conclusion: Treatment with ZYD significantly alleviated paroxetine-induced sexual dysfunction by increasing serum testosterone levels and penile NO, cGMP and eNOS levels, reducing penile PDE5 level.