| 蛇床子总香豆素对新生大鼠成骨细胞的作用 |
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| 引用本文： | 张巧艳,秦路平,郑汉臣,田野苹,黄茅,刘祖德.蛇床子总香豆素对新生大鼠成骨细胞的作用[J].中成药,2001,23(2):111-113. |
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| 作者姓名： | 张巧艳 秦路平 郑汉臣 田野苹 黄茅 刘祖德 |
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| 作者单位： | 第二军医大学药学院,上海,200433 |
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| 基金项目： | 国家自然科学基金项目,批准号39770905 |
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| 摘 要： | 目的：研究蛇床子总香豆素（TCFC）在体外对新生大鼠颅盖骨成骨细胞增殖及成骨作用的影响。方法：酶消化法分离培养新生大鼠颅盖骨成骨细胞；^3H-胸腺嘧啶和^3H-脯氨酸掺入法分别测定细胞增殖和胶原合成；磷酸苯二钠法测定细胞内骨碱性磷酸酶（ALP）活性；并以抗骨质疏松药物异丙黄酮（ipriflavone,IP)作为阳性对照药物。结果：TCFC对新生大鼠成骨细胞的增殖，ALP活性，骨胶原合成具有显的促进作用。结论：TCFC抗骨质惊讶松的作用与其增加成骨细胞的数量，促进细胞胶魇蛋白及ALP的活性有关。
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| 关 键 词： | 蛇床子 香豆素 骨质疏松 成骨细胞 碱性磷酸酶 胶原合成 中医药治疗 新生大鼠 |
| 文章编号： | 1001-1528(2001)02-0111-03 |
| 修稿时间： | 2000年6月26日 |
Effect of Total Coumarins of Fructus Cnidii on Osteoblast in Neonatal Rat Calvaria Culture |
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| ZHANG Qiao yan,QIN Lu ping,ZHENG Han chen,TIAN Ye ping,HUANG Mao,LIU Zu de.Effect of Total Coumarins of Fructus Cnidii on Osteoblast in Neonatal Rat Calvaria Culture[J].Chinese Traditional Patent Medicine,2001,23(2):111-113. |
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| Authors: | ZHANG Qiao yan QIN Lu ping ZHENG Han chen TIAN Ye ping HUANG Mao LIU Zu de |
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| Institution: | ZHANG Qiao yan,QIN Lu ping*,ZHENG Han chen,TIAN Ye ping,HUANG Mao,LIU Zu de |
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| Abstract: | Objective: To study the effects of TCFC on proliferation and osteogenic action of osteoblast in neonatal rat calvaria cultures in vitro. Methods: Osteoblasts were isolated from neonatal rat calvaria through trypsin and collagenase digestion. The proliferation and collagen synthesis were assayed by 3H TdR and 3H proline incorporation. The activity of ALP was measured by p nitrophenyl sodium phosphate assay. The ipriflavone was used as positive control drug.Results: TCFC significantly promoted bone cell proliferation, alkaline phosphatase activity and collage synthesis.Conclusion: TCFC had the anti osteoporosis action by promoting osteoblast proliferation, ALP activity and collage synthesis. |
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| Keywords: | Fructus Cnidii coumarins osteoporosis osteoblast alkaline phosphatase collage synthesis |
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