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桑叶总黄酮的大孔树脂纯化工艺
引用本文:花蕾,张文清,夏玮.桑叶总黄酮的大孔树脂纯化工艺[J].中成药,2007,29(12):1758-1761.
作者姓名:花蕾  张文清  夏玮
作者单位:华东理工大学化学与分子工程学院,上海,200237
摘    要:目的:选择8种大孔吸附树脂分别对桑叶总黄酮进行静态与动态的吸附与解吸,筛选纯化桑叶总黄酮的最佳树脂。方法:研究影响分离的各种因素如流速、上柱浓度、pH和洗脱剂等优化分离工艺。结果:研究结果表明:AB-8树脂宜于桑叶总黄酮的提纯,最佳工艺为:流速为2BV/h,上样桑叶总黄酮水溶液浓度为0.613mg/mL,pH值为4,乙醇为洗脱剂,洗脱浓度为70,树脂每使用3次进行一次再生。结论:经AB-8树脂吸附分离后,黄酮含量提高1倍以上。

关 键 词:大孔树脂  桑叶总黄酮  工艺优化
文章编号:1000-1528(2007)12-1758-04
收稿时间:2007-01-12
修稿时间:2007年1月12日

Purification of total flavones from Mulberry Leaves by macroporous resin
HUA Lei,ZHANG Wen-qing,XIA Wei.Purification of total flavones from Mulberry Leaves by macroporous resin[J].Chinese Traditional Patent Medicine,2007,29(12):1758-1761.
Authors:HUA Lei  ZHANG Wen-qing  XIA Wei
Abstract:AIM:To sieve the optimal purification of the total flavonoid from Mulberry Leaves by exploring 8 types of macroporous resin's static and dynamic adsoption-desorption.METHODS:The factors affecting separation,such as flow rate concentration,pH and kind of eluting agent were considerated.RESULTS:Experiment results showed that AB-8 resin possesses high absorption capacity.The best craft was:the adsorption and desorption-power was 2 BV/h,the content of the total flavonoid in liquid was 0.613 mg per 1 mL,pH 4,as the desorption solvent,the concentration of ethanol was 70%,regenerate the resin after using three times.CONCLUSION:Flavones content used the method can be up 2-fold.
Keywords:macroporous resin  total flavonoes of Mulberry Leaves  optimization of technics
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