HPLC波长切换法同时测定消炎灵片中异嗪皮啶、迷迭香酸和苦玄参苷ⅠA

目的建立同时测定消炎灵片(苦玄参、肿节风、千里光、毛冬青、甘草)中异嗪皮啶、迷迭香酸和苦玄参苷ⅠA含有量的液相色谱法。方法采用高效液相色谱波长切换法同时测定消炎灵片中异秦皮啶、迷迭香酸和苦玄参苷ⅠA。色谱条件为Agilent ZORBAX SB C18(250 mm×4.6 mm,5μm)柱;以乙腈(A)-0.1%磷酸溶液(B)为流动相进行梯度洗脱;体积流量为1.0 mL/min;检测波长前25 min为330 nm,25 min后为264 nm。结果异嗪皮啶在0.018～0.163μg范围内线性关系良好(r=0.999 9,n=6),平均加样回收率为99.8%,RSD为1.8%(n=6);迷迭香酸在0.027～0.244μg范围内线性关系良好(r=0.999 8,n=6),平均加样回收率为99.6%,RSD为1.1%(n=6);苦玄参苷ⅠA在0.271～2.435μg范围内线性关系良好(r=0.999 9,n=6),平均加样回收率为99.6%,RSD为1.4%(n=6)。结论本方法可同时测定消炎灵片中异嗪皮啶、迷迭香酸和苦玄参苷ⅠA,具有快速、简便的特点。

Simultaneous determination of isofraxidin,rosmarinic acid and picfeltarraenin I_A in Xiaoyanling Tablets by HPLC with wavelength switching

AIM To determine simultaneously isofraxidin,rosmarinic acid and picfeltarraenin ⅠA in Xiaoyanling Tablets(Picriae Herba,Sarcandrae Herba,Senecionis scandentis Herba,Ilicis pubescentis Radix,Glycyrrhizae Radix et Rhizoma).METHODS Isofraxidin,rosmarinic acid and picfeltarraenin ⅠA were determined by HPLC simultaneously with changing ultraviolet-visible wavelength.An Agilent ZORBAX SB C18 column(250 mm×4.6 mm,5 μm)was used.The mobile phase A was acetonitrile and the mobile phase B was water with 0.1% phosphoric acid solution in gradient elution mode.The detection wavelength of isofraxidin and rosmarinic acid was set at 330 nm and the detection wavelength of picfeltarraenin ⅠA was set at 264 nm.RESULTS Isofraxidin had a good linearity in the range of 0.018-0.163 μg and its average recovery was 99.8% with RSD of 1.8%.Rosmarinic acid had a good linearity in the range of 0.027-0.244 μg,and its average recovery was 99.6% with RSD of 1.1%.Picfeltarraenin ⅠA had a good linearity in the range of 0.271-2.435 μg,and its average recovery was 99.6% with RSD of 1.4%.CONCLUSION The method is simple,accurate and reproducible.It can be used to determine isofraxidin,rosmarinic acid and picfeltarraenin ⅠA together in Xiaoyanling Tablets with the same chromatogram condition.