| 马钱子碱及生物转化氮氧化物对斑马鱼胚胎毒性的研究 |
| |
| 引用本文： | 张启春,杨雨微,卞慧敏.马钱子碱及生物转化氮氧化物对斑马鱼胚胎毒性的研究[J].中成药,2011,33(10):1664-1668. |
| |
| 作者姓名： | 张启春 杨雨微 卞慧敏 |
| |
| 作者单位： | 南京中医药大学药学院临床药理教研室,江苏南京,210029 |
| |
| 基金项目： | 江苏省中药炮制重点实验室开放式课题(ZYPZ010) |
| |
| 摘 要： | 目的 通过观察斑马鱼胚胎发育的情况,评价马钱子碱及生物转化产物马钱子碱氮氧化物对斑马鱼胚胎毒性的差别.方法 马钱子碱、马钱子碱氮氧化物培养斑马鱼6h受精卵,观察24、96 h死亡情况并计算LC50、孵化率、相对成活率、幼鱼形态变化;细胞凋亡分别采用吖啶橙法及流式细胞仪检测.结果 给药后24、96 h的马钱子碱氮氧化物的LC50分别是马钱子碱的15倍及10倍,同时孵化率及成活率明显高于马钱子碱组;200 μmol/L质量浓度时,马钱子碱组鱼胚胎出现脊柱侧弯,心包水肿等现象,相同质量浓度的马钱子碱氮氧化物组则未观察到该类现象;马钱子碱组斑马鱼细胞凋亡明显,而其氮氧化物组则无明显凋亡.结论 马钱子碱氮氧化物较马钱子碱对斑马鱼胚胎的毒性自明显降低.
|
| 关 键 词： | 斑马鱼 发育毒性 马钱子碱 马钱子碱氮氧化物 |
Embryo toxicity of brucine and biological transformation product brucine nitrogen oxide to zebrafish |
| |
| ZHANG Qi-chun
,
YANG Yu-wei
,
BIAN Hui-min.Embryo toxicity of brucine and biological transformation product brucine nitrogen oxide to zebrafish[J].Chinese Traditional Patent Medicine,2011,33(10):1664-1668. |
| |
| Authors: | ZHANG Qi-chun YANG Yu-wei BIAN Hui-min |
| |
| Institution: | ZHANG Qi-chun,YANG Yu-wei,BIAN Hui-min*(Department of Clinical Pharmacology,College of Pharmacy,Nanjing University of Chinese Medicine,Nanjing 210029,China) |
| |
| Abstract: | AIM The difference of embryo toxicity of brucine and brucine N-oxide to zebrafish through assayed via observing the development of zebrafish embryos osperm.METHODS LC50,hatching rate,relative survival rate and morphologic change were detected in 6 h zebrafish embryos osperm with incubation of brucine and brucine N-oxide for 96 h.Meanwhile,AO staining and flow cytometry were utilized to analyse the apoptose levels.RESULTS The experiment revealed that LC50 of brucine N-oxide was 15 and 10 folds of brucine at ... |
| |
| Keywords: | zebrafish embryo toxicity brucine brucine N-oxide |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
