| 20(S)-人参皂苷Rh2对人结肠癌细胞增殖和周期的影响 |
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| 引用本文： | 李秋影,颜璐璐,马晓慧,张莉华,张兰兰,郭治昕.20(S)-人参皂苷Rh2对人结肠癌细胞增殖和周期的影响[J].中成药,2011,33(11):1874-1878. |
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| 作者姓名： | 李秋影 颜璐璐 马晓慧 张莉华 张兰兰 郭治昕 |
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| 作者单位： | 天津中医药大学;天津天士力研究院药理毒理所; |
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| 基金项目： | 国家科技重大新药创制专项资助项目(2008ZX09401-006) |
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| 摘 要： | 目的研究20(S)-人参皂苷Rh2对人结肠癌Caco-2和HT-29细胞增殖及周期的影响。方法采用荧光微孔法测定20(S)-人参皂苷Rh2对人结肠癌Caco-2、HT-29细胞增殖的影响;利用显微镜观察给药后HT-29细胞形态变化;流式细胞术分析20(S)-人参皂苷Rh2对HT-29细胞周期分布的影响。结果 20(S)-人参皂苷Rh2对Caco-2、HT-29细胞生长有抑制作用,并呈剂量依赖性;20(S)-人参皂苷Rh2作用48 h后,对HT-29、Caco-2细胞的半数抑制浓度(IC50)分别为19.68、26.79μg/mL;流式细胞分析结果显示,与对照组细胞相比,20(S)-人参皂苷Rh2能显著减少HT-29细胞的G0/G1期和G2/M期细胞比例,增加S期细胞比例。结论 20(S)-人参皂苷Rh2可抑制人结肠癌细胞增殖,主要原因是阻滞细胞处于S期。
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| 关 键 词： | 20(S)-人参皂苷Rh2 HT-29 Caco-2 细胞增殖 细胞周期 |
Effect of 20(S)-ginsenoside Rh_2 on cell proliferation and cycle of human colon cancer cells |
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| LI Qiu-ying,YAN Lu-lu,MA Xiao-hui,ZHANG Li-hua,ZHANG Lan-lan,GUO Zhi-xin.Effect of 20(S)-ginsenoside Rh_2 on cell proliferation and cycle of human colon cancer cells[J].Chinese Traditional Patent Medicine,2011,33(11):1874-1878. |
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| Authors: | LI Qiu-ying YAN Lu-lu MA Xiao-hui ZHANG Li-hua ZHANG Lan-lan GUO Zhi-xin |
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| Institution: | LI Qiu-ying1,YAN Lu-lu2,MA Xiao-hui2,ZHANG Li-hua2,ZHANG Lan-lan2,GUO Zhi-xin2(1.Tianjin University of Traditional Chinese Medicine,Tianjin 300193,China,2.Department of Pharmaclogy and Toxicology of Tasly,Tianjin 300410,China) |
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| Abstract: | AIM To investigate the effects of 20(s)-ginsenoside-Rh2 on human colon cancer cells.METHODS Caco-2 and HT-29 human colon cancer cell lines were used.The antiproliferative effects of 20(s)-ginsenoside-Rh2 were assessed by fluorescence microporous assays.Cellular morphological changes were observed under microscopy.The cell cycle was measured by flow cytometry.RESULTS 20(s)-ginsenoside-Rh2 inhibited the proliferation of Caco-2 and HT-29 cell lines in a dose-dependent manner.Caco-2 and HT-29 cell lines were se... |
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| Keywords: | 20(S)-ginsenoside Rh2 HT-29 Caco-2 cell proliferation cell cycle |
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