HPLC-DAD法同时测定四磨汤口服液中5种多酚

目的 建立HPLC-DAD法同时测定四磨汤口服液(槟榔、枳壳、乌药等)中5种多酚的含有量.方法 该药物提取液的分析采用XSelect HSS T3色谱柱(4.6 mm×250 mm,5μm);流动相乙腈-2%乙酸,梯度洗脱;体积流量1.0 mL/min;柱温25℃;检测波长280 nm.结果 焦性没食子酸、表没食子儿茶素、儿茶素、表儿茶素、表没食子儿茶素没食子酸酯分别在7.94~ 158.72、11.96~ 239.28、19.96~399.20、15.90~ 318.08、11.98~ 239.52 μg/mL范围内线性关系良好(R2≥0.999 0),平均加样回收率分别为101.58%、101.74%、100.12%、101.53%、101.12%,RSD分别为1.3%、1.2%、1.7%、0.8%、0.5%.四磨汤口服液中未检测出儿茶素,其他4种成分含有量低于槟榔液中.结论 该方法准确、灵敏、简便,可用于四磨汤口服液的质量控制.

Simultaneous determination of five polyphenols in Simotang Oral Liquid by HPLC DAD

AIM To establish an HPLC DAD method for the simultaneous content determination of five poly phenols in Simotang Oral Liquid(Arecae Semen,Aurantii Fructus,Linderae Radix,etc.).METHODS The anal ysis of extract of this drug was performed on a 25℃thermostatic XSelect HSS T3 column(4.6 mm×250 mm,5μm),with the mobile phase comprising of acetonitrile 2%acetic acid flowing at 1.0 mL/min in a gradient elu tion manner,and the detection wavelength was set at 280 nm.RESULTS Pyrogallic acid,epigallocatechin,cat echin,epicatechin,epigallocatechin gallate showed good linear relationships within the ranges of 7.94-158.72,11.96-239.28,19.96-399.20,15.90-318.08,11.98-239.52μg/mL(R2≥0.9990),whose average recov eries were 101.58%,101.74%,100.12%,101.53%,101.12%with the RSDs of 1.3%,1.2%,1.7%,0.8%,0.5%,respectively.Catechin was not detected in Simotang Oral Liquid,and the other four constituents′contents were lower than those in Arecae Semen solution.CONCLUSION This accurate,sensitive and simple method can be used for the quality control of Simotang Oral Liquid.