HPLC法同时测定健儿膏中8种成分

目的建立HPLC法同时测定健儿膏(党参、山药、甘草等)中白术内酯Ⅲ、白术内酯Ⅰ、党参炔苷、紫丁香苷、去氢土莫酸、土莫酸、去氢茯苓酸、茯苓酸的含有量。方法该药物甲醇提取液的分析采用Diamonsil Plus C18色谱柱(4.6 mm×250 mm,5μm);流动相乙腈-0.05%磷酸,梯度洗脱;体积流量1.0 mL/min;柱温30℃;检测波长210、220、266 nm。结果 8种成分在各组范围内线性关系良好(r≥0.999 1),平均加样回收率96.99%~100.01%,RSD 0.83%~1.47%。结论该方法准确可靠,重复性好,可用于健儿膏的质量控制。

Simultaneous determination of eight constituents in Jian’er Ointment by HPLC

AIM To establish an HPLC method for the simultaneous content determination of atracylenolideⅢ, atracylenolideⅠ, lobetyolin, syringin, dehydrotumulosic acid, tumulosic acid, dehydropachymic acid and pachymic acid in Jian’er Ointment(Codonopsis Radix, Dioscoreae Rhizoma, Glycyrrhizae Radix et Rhizoma, etc.).METHODS The analysis of methanol extract of this drug was performed on a 30 ℃ thermostatic Diamonsil Plus C18 column(4.6 mm×250 mm, 5 μm), with the mobile phase comprising of acetonitrile-0.05% phosphoric acid flowing at 1.0 mL/min in a gradient elution manner, and the detection wavelengths were set at 210, 220, 266 nm.RESULTS Eight constituents showed good linear relationships within their own ranges(r≥0.999 1), whose average recoveries were 96.99%-100.01% with the RSDs of 0.83%-1.47%.CONCLUSION This accurate, reliable and reproducible method can be used for the quality control of Jian’er Ointment.