茄根含氮类成分及其抗炎活性

目的研究茄Solanum melongena L.根中含氮类成分及其抗炎活性。方法茄根70%乙醇提取物采用MCI、硅胶、ODS、Sephadex LH?20及HPLC等进行分离纯化,根据理化性质及波谱数据鉴定所得化合物的结构。采用LPS诱导RAW264??7细胞模型评价其抗炎活性。结果从中分离得到12个含氮类化合物,分别鉴定为N?acetyltyramine(1)、N?(2?phenylethyl)acetamide(2)、3?(4?aminophenyl)?prop?2?enoic acid(3)、callyspongi?dipeptide A(4)、pistaciamide(5)、tatarine C(6)、thymidine(7)、butyl?2?pyrrolidone?5?carboxylate(8)、indazole(9)、cyclo?(S)?pro?(R)?leu](10)、seco?(S)?pro?(R)?val](11)、(L)?pro?(L)?phe(12)。化合物4、8、10~12对LPS诱导RAW264.7产生NO具有良好的抑制作用,其IC50值分别为(33.7±1.4)、(59.1±4.6)、(28.6±2.1)、(44.7±3.1)、(52.8±3.8)μmol/L。结论所有化合物均为首次从该植物中分离得到,且化合物4、8、10~12具有潜在的抗炎活性。

Nitrogenous constituents from the roots of Solanum melongena and their anti?inflammatory activities

AIM To study the chemical constituents from the roots of Solanum melongena L.and their anti?inflammatory activities.METHODS The 70%ethanol extract from the roots of S.melongena was isolated and purified by MCI,silica,ODS,Sephadex LH?20 and HPLC,then the structures of obtained compounds were identified by physicochemical properties and spectral data.Their anti?inflammatory activities were estimated by LPS?induced RAW264.7 cell model.RESULTS Twelve nitrogenous compounds were isolated and identified as N?acetyltyramine(1)、N?(2?phenylethyl)acetamide(2)、3?(4?aminophenyl)?prop?2?enoic acid(3)、callyspongi?dipeptide A(4)、pistaciamide(5)、tatarine C(6)、thymidine(7)、butyl?2?pyrrolidone?5?carbox?ylate(8)、indazole(9)、cyclo?((S)?pro?(R)?leu)(10)、seco?((S)?pro?(R)?val)(11)、(L)?pro?(L)?phe(12).Compounds 4,8,10-12 had significant inhibitory effects on NO,with IC50 values of(33.7±1.4),(59.1±4.6),(28.6±2.1),(44.7±3.1),(52.8±3.8)μmol/L,respectively.CONCLUSION All the compounds are isolated from this plant for the first time,and compounds 4,8,10-12 have potential anti?inflammatory activities.