HPLC法同时测定复方川参胶囊中7种成分

目的建立HPLC法同时测定复方川参胶囊(川芎、人参、红花等)中羟基红花黄色素A、细叶远志皂苷、阿魏酸、人参皂苷Re、人参皂苷Rg1、人参皂苷Rb1、人参皂苷Rb2的含量。方法该药物70%甲醇提取液的分析采用Intersil C₁₈色谱柱(250 mm×4.6 mm,5μm);流动相乙腈-水(含1.0 g/L磷酸),梯度洗脱;体积流量0.8 mL/min;柱温35℃;检测波长203、321 nm。结果各成分在各组范围内线性关系良好(R²≥0.9995),平均加样回收率96.00%~100.00%,RSD小于2%。结论该方法重复性好,专属性强,稳定可靠,可用于复方川参胶囊的质量控制。

Simultaneous determination of seven constituents in Compound Chuanshen Cap-sules

AIM To establish an HPLC method for the simultaneous content determination of hydroxy safflower yellow A,tenuifolin,ferulic acid,ginsenoside Re,ginsenoside Rg1,ginsenoside Rb1 and ginsenoside Rb2 in Compound Chuanshen Capsules(Chuanxiong Rhizoma,Ginseng Radix et Rhizoma,Carthami Flos,etc.).METHODS The analysis of 70%methanol extract of this drug was performed on a 35℃thermostatic Intersil C 18 column(250 mm×4.6 mm,5μm),with the mobile phase comprising of acetonitrile-water(containing 1.0 g/L phosphoric acid)flowing at 0.8 mL/min in a gradient elution manner,and the detection wavelengths were set at 203,321 nm.RESULTS Seven components showed good linear relationships within their own ranges(R 2≥0.9995),whose average recoveries were 96.00%-100.00%with the RSDs of less than 2%.CONCLUSION This reproducible,specific,stable and reliable method can be used for the quality control of Compound Chuanshen Capsules.