复方感冒灵颗粒抗流感相关药效作用研究

目的 建立复方感冒灵颗粒（CGG）全方、化学药（CM）组、中药（TCM）组的抗病毒、抑菌、解热、抗炎等实验，以期综合评价复方感冒灵颗粒治疗流行性感冒的药效作用，为临床用药提供参考。方法 分别采用甲型流感病毒制备小鼠病毒感染模型，金黄色葡萄球菌制备小鼠细菌感染模型，酵母菌致大鼠发热模型及巴豆油致小鼠耳肿胀模型，对CGG、CM、TCM的抗病毒、体内体外抑菌、解热和抗炎的作用效果进行研究。结果 小鼠感染流感病毒后，给药组肺组织中病毒载量较模型组明显降低（P<0.05）；CGG、CM、TCM组小鼠的肺指数抑制率分别为19.68%、8.30%、8.54%；各组存活时间较模型组明显延长（P<0.05），延长率分别为29.6%、5.4%、12.9%；病毒感染致小鼠死亡抑制率分别为26.7%、0、10.0%。在体内注射金黄色葡萄球菌感染小鼠致死实验中发现，CGG、CM、TCM对小鼠的死亡抑制率分别为10.0%、15.0%、0；体外抑菌和杀菌实验发现CGG、TCM对金黄色葡萄球菌等标准株均显示出抑制和杀灭作用。解热实验中发现CGG、CM可在给药后1、2 h显著抑制酵母致大鼠发热反应（P<0.01）。抗炎实验中发现给药组耳肿胀值明显低于模型组（P<0.05），CGG、CM、TCM组抑制率分别为31.7%、22.6%、26.8%。结论 CGG具有抗病毒、抑菌、解热、抗炎等功效，且CGG效果优于TCM、CM，提示TCM和CM发挥了协同作用，可用于治疗感冒引起的头痛、发热、鼻塞、流涕、咽痛等上呼吸道感染疾病。

Anti-influenza Efficacy of Compound Ganmaoling Granules

Objective To evaluate the anti-influenza efficacy of Compound Ganmaoling Granules (CGG) and provide a basis for the clinical treatment of common cold.Methods The antiviral, antimicrobial, antipyretic, and anti-inflammatory effects of CGG, chemical medicine (CM), and Chinese herbal medicine (TCM) were detected on the basis of the mouse models infected by influenza A virus, the mouse model infected by Staphylococcus aureus, the rat fever model induced by yeast, and the mouse ear swelling model induced by croton oil.Results For the mice infected with influenza A virus, CGG, CM, and TCM lowered the virus load in the lung tissue (P<0.05), decreased the lung index by 19.68%, 8.30%, and 8.54%, prolonged the mouse survival time by 29.6%, 5.4%, and 12.9% (P<0.05), and decreased the mortality rate by 26.7%, 0, and 10.0%, respectively. For the mice infected with S. aureus, CGG, CM, and TCM decreased the mortality rate by 10.0%, 15.0%, and 0. Moreover, the in vitro experiments indicated that CGG and TCM had inhibitory and killing effects on S. aureus. CGG and TCM can inhibit the fever induced by yeast in rats 1 h and 2 h after administration (P<0.01). CGG, CM, and TCM lowered the ear swelling scores by 31.7%, 22.6%, and 26.8%, respectively, compared with that of the model group (P<0.05).Conclusion CGG has antiviral, antimicrobial, antipyretic and anti-inflammatory activities superior to CM and TCM, which can be used to treat headache, fever, nasal obstruction, running nose, and sore throat caused by upper respiratory tract infections.