金威消石颗粒对小鼠镇痛作用的研究△

目的:探索金威消石颗粒对小鼠痛阈值的影响及对醋酸致小鼠扭体反应的作用。方法:ICR小鼠60只随机分入金威消石颗粒低、中、高剂量组、阳性药组和对照组,分别经胃灌注不同剂量金威消石颗粒、双氯芬酸钠溶液和蒸馏水,以平板法检测各小鼠给药前及给药后1,2,3,5h的痛阈值。另取ICR小鼠60只,随机分入上述5组,以上法经胃灌注上述液体,每日1次,连续7d。于末次给药后1h,各小鼠由腹腔注射0.7%醋酸溶液0.2mL,计数注射后20min内各小鼠的扭体次数。结果:金威消石颗粒各剂量组在给药后2,3,5h时的痛阈值均明显高于对照组(P<0.01);各剂量组3h和5h痛阈值明显高于各组给药前基础痛阈值和1h痛阈值(P<0.01)。金威消石颗粒各剂量组醋酸致小鼠的平均扭体次数均明显低于对照组(P<0.01)。结论:金威消石颗粒可提高ICR小鼠热板法痛阈值,减少醋酸致小鼠的扭体次数,具有明显的镇痛作用。

Analgesic Effect of Jinwei Lithiasis-eliminating Granules in Mice

Objective： To explore the influence of Jinwei lithiasis-eliminating granules on the pain threshold of mire and its effect on torsion numbers of acetic acid-induced mice. Methods： Sixty ICR mice were randomly distributed to three pilot groups（low-, middle-and high-dose group）, positive drug group and control group. Aqueous solutions containing Jinwei lithiasis-eliminating granules （ 1.33, 2. 67, 5.32 g . kg -1 ）, diclofenac sodium tablet （ 16.6 mg.kg-I ） and isovolemic distilled water were plate method was used to measure the pain thresholds perfused by stomach for corresponding mice, respectively, and of mice before and I, 2, 3, 5 h after stomach perfusion in each group. Another 60 mice were randomly distributed to the above-mentioned groups and managed by the same stomach perfusion, once daily, for 7 d. Each mouse was injected intra-peritoneally 0. 2 mL of 0. 7% acetic acid solution 1 h just after the final perfusion, and the torsion numbers of all mice during post-injection twenty minutes＇ period was counted. Results： The mean pain thresholds of mice 2, 3, 5 h after stomach perfusion were significantly higher in three pilot groups than that in control group（P 〈0.01 ）, whereas no significant difference when compared to positive drug group （ P 〉 0. 05 ）. The mean pain thresholds of mice 3 h and 5 h after stomach perfusion were significantly higher than those before and 1 h after stomach perfusion in the pilot groups（P 〈0. 01 ）. The mean torsion numbers of mice in three pilot groups were less than that in control group（P 〈0.01 ）, no significant difference existed in the mean torsion numbers of mice between three pilot groups and positive drug group （ P 〉 0. 05 ） . Conclusion： Jinwei lithiasis- eliminating granules could increase the pain threshold of ICR mice by plate method and decrease the torsion numbers in acetic acid-induced mice, implying a considerable analgesic effect.