HPLC-ELSD同时测定鲜竹沥中单糖双糖的含量

目的:建立同时测定鲜竹沥中主要单糖和双糖(果糖、葡萄糖、蔗糖)含量的高效液相色谱-蒸发光散射检测器(HPLC-ELSD)方法。方法:采用LunaAOmega SUGAR 100A(250 mm×4.6 mm,3μm)色谱柱,流动相为乙腈-水(85∶15),流速为1.0 mL·min^-1,柱温为35℃,ELSD漂移管温度为70℃,N2流速为2.0 L·min^-1。结果:果糖、葡萄糖和蔗糖分别在0.8136~4.0678(r=0.9994)、1.0569~5.2847(r=0.9996)、1.0938~5.4690μg(r=0.9996)其对数值与峰面积的对数值呈良好的线性关系;平均加样回收率分别为98.1%、91.9%、99.2%,RSD分别为1.3%、0.9%、1.1%。结论:本法经过方法学验证,可用于鲜竹沥中主要单糖和双糖的质量控制。

Simultaneous Quantification of Monosaccharides and Disaccharides in Xianzhuli by HPLC-ELSD

Objective:To establish the method of high performance liquid chromatography-evaporative light-scattering detector(HPLC-ELSD)for simultaneous quantification of monosaccharides and oligosaccharides(fructose,glucose and sucrose)in Xianzhuli.Methods:A chromatographic column of lunaA Omega SUGAR 100A(250 mm×4.6 mm,3 μm)was adopted.The mobile phase was the mixture of acetonitrile-water(85∶15) at a flow rate of 1.0 L·min-1 with the column temperature set at 35 ℃.The drift tube temperature was taken at 70 ℃,the carrier gas flow rate at 2.0 L·min-1.Results:Fructose,glucose and sucrose showed a good linear relationship between the logarithmic values of content and peak area within 0.813 6-4.067 8(r=0.999 4),1.056 9-5.284 7(r=0.999 6) and 1.093 8-5.469 0 μg(r=0.999 6),and the average recovery rates were 98.1%(RSD=1.3%),91.9%(RSD=0.9%) and 99.2%(RSD=1.1%),respectively.Conclusion:The method has been validated.It can be used for quality control of simultaneous quantification of monosaccharides and oligosaccharides in Xianzhuli.