临泉产穿心莲地上部分黄酮类化学成分研究

目的:研究临泉产穿心莲地上部分黄酮类化学成分。方法:利用硅胶、凝胶Sephadex LH-20、ODS柱色谱等方法,对临泉产穿心莲地上部分90%乙醇提取物的化学成分进行分离和纯化,运用波谱学技术对分离得到的单体化合物进行结构鉴定。结果:从穿心莲的乙醇提取物中分离并鉴定出14个黄酮类化合物,分别是双氢汉黄芩素(1)、5-羟基-6,7-二甲氧基黄酮(2)、2-羟基-3,4,6-三甲氧基查尔酮(3)、5-羟基-7,8-二甲氧基黄酮(4)、穿心莲素(5)、5-羟基-7,8,2',5'-四甲氧基黄酮(6)、5,7,8-三甲氧基二氢黄酮(7)、2'-甲氧基-5,6,7-三甲基黄芩素(8)、5,7,4'-三甲氧基黄酮(9)、高车前素(10)、7,8,2',5'-四甲氧基黄酮-5-O-β-D-吡喃葡萄糖苷(11)、穿心莲黄酮苷A(12)、穿心莲黄酮苷C(13)、穿心莲黄酮苷G(14)。结论:其中化合物1、2、3、8首次从穿心莲中分离得到。

Studies on the Chemical Constituents of Flavonoids in the Aerial Parts of Andrographis paniculuta from Linquan

Objective:To study the chemical constituents of flavonoids in the aerial parts of Andrographis paniculuta(Burm.f)Nees from Linquan.Methods:The 90%ethanol extract in the aerial parts of A.paniculata were separated and purified by repeated silica gel,Sephadex LH-20 and ODS column chromatographies,and the structures of the isolates were identified by spectroscopic techniques.Results:Fourteen flavonoids were isolated and identified from the plant.These compounds were determined as dihydrowogonin(1),5-hydroxy-6,7-dimethoxyflavone(2),2-hydroxy-3,4,6-trimethoxychalcone(3),5-hydroxy-7,8-dimethoxyflavone(4),panicolin(5),5-hydroxyl-7,8,2',5'-tetramethoxyflavones(6),5,7,8-trimethoxydihydroflavone(7),2'-methoxy-5,6,7-trimethylbaicalein(8),5,7,4'-trimethoxyflavone(9),hispidulin(10),7,8,2',5'-tetramethoxyflavone-5-O-β-D-pyranoglucoside(11),andrographidine A(12),andrographidine C(13),andrographidine G(14).Conclusion:Compounds 1,2,3,8 were isolated from the A.paniculata for the first time.