银线草化学成分研究

目的:对金粟兰属植物银线草Chloranthus japonicus Sieb.全草的化学成分进行研究,以探究其药理活性物质基础。方法:采用硅胶、凝胶Sephadex LH-20、半自动制备液相等色谱方法对银线草的乙酸乙酯部位进行分离纯化,通过1D-NMR、紫外(UV)光谱、质谱(MS)技术对化合物的结构进行鉴定。结果:从银线草75%乙醇提取物的乙酸乙酯部位共分离得到9个化合物,分别鉴定为金合欢素(1)、千层纸素A(2)、7 E-3,4-二氧甲基苯并2-苯基-胸苷](3)、落叶松脂素(4)、9α-羟基紫菀内酯(5)、银线草醇B(6)、金粟兰素C(7)、白术内酯Ⅲ(8)、白术内酯Ⅱ(9)。结论:化合物14为首次在金粟兰属中分离得到。

Study on Chemical Constituents of Chloranthus japonicus

Objective:To study chemical constituents of the whole plant of Chloranthus japonicus Sieb.and explore the basis of pharmacological substances.Methods:The compounds were isolated and purified by repeated silica gel,gel Sephadex LH-20 and semi-automatic preparative liquid chromatography form EtOAc extraction of C.japonicus.The structure of the compound was identified by 1D-NMR,UV and MS.Results:Nine compounds were isolated,which were identified as acacetin(1),6-methoxybaicalein(2),7 E-3,4-dioxymethylenzimtsaure-2-phenyle-thylamid](3),lariciresinol(4),9α-hydroxyasterolide(5),shizukaol B(6),spicachlorantin C(7),atractylenolideⅢ(8),atractylenolideⅡ(9).Conclusion:Compounds 14 were isolated from japonicus for the first time.