HPLC同时测定归芍疏肝散中芍药苷阿魏酸和甘草酸铵的含量

目的:建立同时测定归芍疏肝散中芍药苷、阿魏酸和甘草酸铵含量的高效液相色谱法。方法:采用Amethyst C18色谱柱(200 mm×4.6 mm,5μm),乙腈-0.1%磷酸为流动相,梯度洗脱,流速为1.0 mL·min-1,检测波长为237 nm,柱温为30℃。结果:在线性范围内3种成分线性良好(r≥0.9998),精密度RSD均<2%;重复性RSD均<3%;平均回收率98.81%~100.25%,RSD均<2%。结论:该方法快捷,操作简便,结果可靠,可用于归芍疏肝散的质量控制。

Simultaneous Determination of Paeoniflorin,Ferulic Acid and Ammonium Glycyrrhizinate in Guishao Shugan Powder by HPLC

Objective:To establish an HPLC method for simultaneous determination of paeoniflorin,ferulic acid and ammonium glycyrrhetate in Guishao Shugan powder.Methods:The HPLC assay carried out on an Amethyst C18 chromatographic column(200 mm×4.6 mm,5μm)by a gradient elution using acetonitrile-0.1%phosphoric acid.The detection wavelength was set at 237 nm,flow rate at 1.0 mL·min^-1 and column temperature at 30℃.Results:The established method had good linearity(r≥0.9998),precision(RSD<2%),repeatability(RSD<3%)and accuracy due to all average recoveries ranged was 98.81%-100.25%(RSD<2%).Conclusion:This method is fast,simple and reliable,which can be used for quality control of Guishao Shugan powder.