| 麦冬及其混伪品的ITS2序列分析及鉴别研究 |
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| 引用本文： | 郑司浩,孙稚颖,黄林芳.麦冬及其混伪品的ITS2序列分析及鉴别研究[J].中国现代中药,2012,14(1):21-23. |
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| 作者姓名： | 郑司浩 孙稚颖 黄林芳 |
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| 作者单位： | 1. 中草药物质基础与资源利用教育部重点实验室 中国医学科学院 北京协和医学院药用植物研究所,北京,100193
2. 山东中医药大学药学院,山东 济南,250355 |
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| 基金项目： | 国家自然科学基金重点项目（30530860） |
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| 摘 要： | 目的:对麦冬及其易混伪品进行分子鉴别,以确保该药材的质量和临床安全用药.方法:从GenBank数据库中下载麦冬及其混伪品的ITS2片段序列,利用MEGA等软件进行相关数据分析,构建NJ树,并预测麦冬及其混伪品的ITS2二级结构.结果:麦冬与其3个混伪品的ITS2序列间存在明显差异,基于ITS2序列的NJ树及其二级结构亦能对麦冬及其混伪品进行鉴别.结论:ITS2条形码序列可有效地鉴别麦冬及其混伪品.
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| 关 键 词： | 麦冬 混伪品 ITS2 分子鉴定 DNA条形码 |
| 收稿时间： | 2011/11/23 0:00:00 |
Analysis and Identification of Ophiopogon japonicus and Its Adulterants Using ITS2 Sequences |
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| ZHENG Si-hao,SUN Zhi-ying and HUANG Lin-fang.Analysis and Identification of Ophiopogon japonicus and Its Adulterants Using ITS2 Sequences[J].Modern Chinese Medicine,2012,14(1):21-23. |
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| Authors: | ZHENG Si-hao SUN Zhi-ying and HUANG Lin-fang |
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| Institution: | 1(1.The Key 4Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine,Ministry of Education Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences,Peking Union Medical College,Beijing 100193 China;2.College of Chinese Medicine,Shandong University of Traditional Chinese Medicine,Jinan 250355,China) |
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| Abstract: | Objective:To analysis the DNA barcodes of Ophiopogonis Radix and its adulterants,and to ensure the quality and clinical safety of drugs.Methods:Download the ITS2 sequences of Ophiopogonis Radix and its adulterants from GenBank database,and the genetic distances were computed using MEGA 4.0 in accordance with the Kimura 2-Parameter(K2P) model,and the phylogenetic tree was constructed.The ITS2 secondary structure was predicted using the ITS2 database and website founded by Schultz et al.Results:Ophiopogonis Radix and its adulterants can be identified by the differences of the ITS2 sequences.The phylogenetic tree and the ITS2 secondary structure can also distinguish between Ophiopogonis Radix and its adulterants.Conclusion:ITS2 barcode sequences can serve as a valuable marker to identifying Ophiopogonis Radix and its adulterants,and it has a broad application prospects in the field of Traditional Chinese Medicine. |
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| Keywords: | Ophiopogon japonicus Adulterants ITS2 Molecular identification DNA barcoding |
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