| 高速逆流色谱法分离纯化浙贝母中贝母素甲, 贝母素乙 |
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| 引用本文： | 金艳,沈平孃,张继全,卓超,徐超,俞雁.高速逆流色谱法分离纯化浙贝母中贝母素甲, 贝母素乙[J].中国现代中药,2005,7(12):13-15. |
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| 作者姓名： | 金艳 沈平孃 张继全 卓超 徐超 俞雁 |
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| 作者单位： | 1. 华东理工大学,上海,200237
2. 国家中药制药工程研究中心,上海,201203 |
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| 摘 要： | 目的:采用高速逆流色谱技术分离提纯浙贝母中的活性成分贝母素甲,贝母素乙.方法:采用氯仿-无水乙醇-0.2mo1.L-1盐酸(4:3:2)系统,上相为固定相,下相为流动相.主机转速为800r.min-1,流动相流速为1.2mL·min-1.结果:一次进样分离得到贝母素甲、贝母素乙,经高效液相(HPLC)分析纯度,经MS,1HNMR鉴定结构.结论:利用本实验条件可从浙贝母中分离制备贝母素甲,贝母素乙,两者纯度分别达到:97.5%,94.5%.
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| 关 键 词： | 高速逆流色谱 浙贝母 贝母素甲 贝母素乙 |
| 收稿时间： | 2005/10/24 0:00:00 |
| 修稿时间： | 2005年10月24 |
Separation and Purification of peimine and peiminine from Bulbus Fritillariae Thunbergi by High Speed Countercurrent Chromatography |
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| Jin Yan,Shen Pingniang,Zhang Jiquan,Zhuo Chao,Xu Chao and Yu Yan.Separation and Purification of peimine and peiminine from Bulbus Fritillariae Thunbergi by High Speed Countercurrent Chromatography[J].Modern Chinese Medicine,2005,7(12):13-15. |
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| Authors: | Jin Yan Shen Pingniang Zhang Jiquan Zhuo Chao Xu Chao and Yu Yan |
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| Institution: | East China University of Science and Technology, Shanghai, 200237, China;;National Engineering Research Center for Traditional Chinese Medicine, Shanghai, 201203, China;National Engineering Research Center for Traditional Chinese Medicine, Shanghai, 201203, China;East China University of Science and Technology, Shanghai, 200237, China;;East China University of Science and Technology, Shanghai, 200237, China;;East China University of Science and Technology, Shanghai, 200237, China; |
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| Abstract: | Objective:High Speed Countercurrent chromatography was applied to separation and purification of peimine and peiminine from Bulbus Fritillariae Thunbergi.Methods:The solvent system used for HSCCC was chloroform-ethanol -0.2mol·L-1 hydrochloric acid(V/V,4:3:2),the organic layer was used as stationary phase,the aqueous phase was used as mobile phase.The rotational speed of main engine was 800 r·min-1,the flow of mobile phase was 1.2 mL·min-1.Result:The purified peimine and peiminine were dentified and analyzed by HPLC,1HNRM,MS.Conclusions:Under this condition peimine and peiminine can be separated and purified from Bulbus Fritillariae Thunbergi.purities were respectively 97.5% and 94.5%. |
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| Keywords: | HSCCC Bulbus Fritillariae Thunbergi peimine peiminine |
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