水蛭的高效液相指纹图谱研究

目的以黄嘌呤和次黄嘌呤为对照品,系统的完成水蛭指纹图谱的建立。方法采用Agilent TC-C18色谱柱(250 mm×4.6 mm,5μm),流动相0.01 mol/L磷酸二氢钾-甲醇梯度洗脱,检测波长254 nm,流速0.8 ml/min,柱温25℃,进样量20μl。结果 10批水蛭药材的色谱指纹图谱得到10个共有峰,其中两个为黄嘌呤和次黄嘌呤的色谱峰。利用药典委员会颁布的《中药色谱指纹图谱相似度评价系统2004A》进行评价,高效液相指纹图谱的相似度在0.669~0.994之间。结论所建立的高效液相指纹图谱可有效地鉴定水蛭,该方法为水蛭药材的质量评价提供了实验数据基础。

HPLC fingerprint study of Hirudo

Objective To establish the fingerprinting of Hirudo systematically,using xanthine and hypoxanthine as reference substances.Method Using Agilent TC-C18（250 mm × 4.6 mm,5μm）;mobile phase A 0.01 mol / L Potassium dihydrogen phosphate,B Methanol,the detection wavelength was set at 254 nm,the flow rate was 0.8 ml / min the column temperature was 25 ℃,the injection volume was 20μl.Result 10 characteristic peaks were identified with the analysis of 10 batches of Hirudo from different habitats,and two of them were Xanthine and Hypoxanthine.According to the similarity evaluation system for Chromatographic Fingerprint of Traditional Chinese Medicine（Version 2004A）,the similarity of the chromatographic fingerprints was between 0.669-0.994.Conclusion HPLC fingerprint can effectively identify Hirudo.The method could provide basis of the experimental data for the quality assessment of Hirudo.