| 砂仁及其混淆品的ITS2序列鉴定 |
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| 引用本文： | 韩建萍,李美妮,石林春,姚辉,宋经元.砂仁及其混淆品的ITS2序列鉴定[J].环球中医药,2011,4(2):99-102. |
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| 作者姓名： | 韩建萍 李美妮 石林春 姚辉 宋经元 |
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| 作者单位： | 中国医学科学院,北京协和医学院药用植物研究所,北京,100193 |
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| 摘 要： | 目的 应用内转录第二间隔区(ITS2)序列及其二级结构对砂仁混淆品进行鉴定.方法应用CLUSTALX1.83 软件进行序列比对,比对后的长度为252 bp.用MEGA 4.1计算种间、种内遗传距离,并构建系统进化树.结果ITS2序列可以将砂仁的三个来源阳春砂Amomum villosum、绿壳砂Amomum villo...
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| 关 键 词： | 砂仁 内转录第二间隔区 DNA条形码 |
Identification of Amomi fructus and its adulterants based on ITS2 sequences |
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| Institution: | HAN Jian-ping,LI Mei-ni,SHI Lin-chun,et al.Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences ＆Peking Union Medical College,Beijing 100193,China |
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| Abstract: | Objective Identification of Amomi fructus and its adulterants based on ITS2 primary sequence and secondary structure.Method CLUSTALX 1.83 software was used to align the sequences and the length of the aligned sequence was 252 bp.The MEGA4.1 software was used to calculate the K-2P distance between intra-and inter-specific species.The NJ tree was also been built.Result The ITS2 sequence would be used to identify Amomum villosum,Amomum villosum var.xanthioides,Amomum longiligulare and its adulterants,such as Alpinia zerumbet,Alpinia japonica,Amomum tsao-ko etc.Conclusion ITS2 sequence was recommended as barcode to identify Amomi fructus and its adulterants. |
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| Keywords: | Amomi fructus ITS2 DNA barcode |
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