| DNA分子标记技术在龙胆鉴别与亲缘关系划分上的应用 |
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| 引用本文： | 曹秀明,郑蔚虹,尚明.DNA分子标记技术在龙胆鉴别与亲缘关系划分上的应用[J].中华中医药学刊,2007,25(4):829-830. |
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| 作者姓名： | 曹秀明 郑蔚虹 尚明 |
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| 作者单位： | 1. 哈尔滨商业大学药物研究所,黑龙江,哈尔滨,150076
2. 温州大学生命与环境学院,浙江,温州,325000 |
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| 摘 要： | 研究东北产药材龙胆的鉴别方法及其亲缘关系，用分子生物学技术对东北产药材龙胆的3个不同品种进行了DNA提取和RAPD及ISSR分析。应用RAPD法从80个随机引物中找出了3种引物（S-76，S-69，S-64）来标记3种龙胆发现这3种引物能把供试的3种龙胆全部区分开。同时应用ISSR法从7个引物中筛选出4个引物能扩增出稳定遗传多态性引物，共扩增出44条DNA条片段，其中同源片段有22个，特异片段有12个，多态片段为10个。用这些引物扩增出的指纹图谱进行聚类分析，得出了相同的结论，即在3种龙胆中，条叶龙胆，龙胆亲缘关系较近，三花龙胆与二者亲缘关系较远。结果表明分子标记技术可用于龙胆的鉴别，亲缘关系划分。
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| 关 键 词： | 龙胆 亲缘关系 分子标记 中药鉴定 |
| 文章编号： | 1673-7717（2007）04-829-02 |
| 收稿时间： | 2006-11-14 |
| 修稿时间： | 2006-11-14 |
Evaluation of the Identification and Phylogeny Relationship among Gentianas by Molecular Markers |
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| CAO Xiu-ming,ZHENG Wei-hong,SHANG Ming.Evaluation of the Identification and Phylogeny Relationship among Gentianas by Molecular Markers[J].Chinese Archives of Traditional Chinese Medicine,2007,25(4):829-830. |
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| Authors: | CAO Xiu-ming ZHENG Wei-hong SHANG Ming |
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| Institution: | 1. Institute of Metefia Medica Harbin University of Commerce Co. Ltd. , Harbin 150076, Heilongjiang, China; 2. Wenzhou University Life and Environment Collage Co. Ltd. ,Wenzhou 325000 ,Zhejiang,China |
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| Abstract: | Random amplified polymorphic DNA(RAPD)and inter simple sequencerepeat(ISSR) methods were applied to detect genetic variation of three kinds of Gentianas source of Northeast area.Eighty primers of RAPD were screened and of which three polymorphic and informative patterns were selected to determine the genetic diversity and phylogeny relationship.At the same time,seven primers of were screened and three of them were selected.Total 44 DNA bands were amplified.10 of which were polymorphic.The results indicated that experiment technology of molecular markers could be used to evaluate the phylogenetic relationship and identify Gentianas. |
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| Keywords: | Gentiana phylogenetie relationship molecular markers identification |
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