艾灸对实验性RA家兔滑膜细胞MAPK信号通路影响的研究

目的：观察艾灸对实验性RA家兔滑膜细胞MAPK信号通路的影响，揭示艾灸治疗实验性RA的分子信号机制。方法：运用基因芯片及生物信息分析技术检测分析MAPK信号通路中96种相关信号分子的表达。结果：造模组与正常对照组相比较，MAPK通路异常激活，Cyclin A1、p38 MAPK、ERK1、JNK1、N—ras等相关信号分子表达上调；艾灸组与造模组相比较，MAPK通路中异常激活的Cyclin A1、p38 MAPK、ERK1、JNK1、N—ras等相关信号分子的表达明显下调。结论：艾灸对实验性RA滑膜细胞MAPKs信号通路的异常激活有明显抑制作用。

The Influence on MAPK Signal Pathway in RA Synovial Cell of Experimental Rabbits by Moxibustion

Objective:Observe the influence of MAPK pathway's signal transduction in RA synovial cell of experimental rabbits by moxibustion,and reveal the molecular signal mechanism of experimental RA by moxibustion.Methods:Apply gene chip inspection and bioinformation analytical technique to detect the 96 sorts of signal molecule of MAPK pathway.Results: Comparing model group with normal group,MAPK pathway has been activated abnormally,and as a result,the expression of signal molecule such as Cyclin A1,p38 MAPK,ERK1,JNK1,Nras has been up-regulated;comparing moxibustion group with model group,the expression of signal molecule such as Cyclin A1,p38 MAPK,ERK1,JNK1,N-ras has been down-regulated after therapy.Conclusion:Moxibustion plays a very important roll of transparent inhibition in abnormal activation of MAPK pathway's signal transduction in RA synovial cell of experimental rabbits.