桑枝对小鼠脑缺血再灌注损伤的保护作用

目的:观察桑枝对小鼠脑缺血再灌注损伤的保护作用。方法:将小鼠分为4组,假手术组;模型组(分离两侧颈总动脉及迷走神经,用微型动脉夹同时夹闭双侧颈总动脉,制作小鼠脑缺血再灌注模型);阳性药组给以尼莫地平24mg/kg,受试药组给以桑枝水提醇沉液14g生药/kg,均灌胃给药7天,24h后取材,匀浆,测脑组织Evan's蓝含量及抗氧化酶SOD活性与MDA水平的变化。结果:缺血再灌注后,脑组织Evan's蓝含量升高,给药后降低。脑缺血再灌注组SOD活性降低,MDA含量增高,而治疗组SOD活性增高,MDA水平降低。结论:桑枝能增加脑缺血再灌注小鼠SOD的活性,改善脑血流,减轻血脑屏障的损伤,对脑缺血再灌注损伤具保护作用。

Protection Effect of Mulberry on Cerebral Ischemia-Reperfusion Injury in Mice Brain Tissue

Objective:To investigate the protection of Mulberry on cerebral ischemia-reperfusion injury in mice brain tissue.Methods:The mice were divided into four groups:control group;model group(separate carotid artery and the vagus nerve,use micro-artery occlusion folder on bilateral carotid artery,reproduce mice brain ischemia-reperfusion model),positive drug group(nimodipine 20mg/kg);tested drug Mulberry group(20mg/kg).Every group took intragastric administration,once a day for seven days.Brain tissue Evan’s Blue content and antioxidant enzymes and other changes were detected.Result:Evan’s Blue content in the brain tissue increased in model group,meanwhile,it decreased after nimodipine and Mulberry administration.SOD activity of cerebral ischemia-reperfusion group decreased and MDA levels increased,but in the treatment group the activity of SOD increased,and MDA decreased.Conclusion:Mulberry can increase the activity of SOD,improve cerebral blood flow and reduce the blood-brain barrier damage in cerebral ischemia-reperfusion of mice.