| 乔松素在大鼠体内外代谢产物的UPLC-MS/MS鉴定 |
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| 引用本文： | 李元圆,赵婷,徐冲,王峥涛.乔松素在大鼠体内外代谢产物的UPLC-MS/MS鉴定[J].中华中医药学刊,2012(4):752-755. |
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| 作者姓名： | 李元圆 赵婷 徐冲 王峥涛 |
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| 作者单位： | 浙江省医学高等专科学校;上海中医药大学教育部中药标准化重点实验室;上海中药标准化研究中心 |
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| 基金项目： | 浙江省教育厅资助项目(Y200909605) |
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| 摘 要： | 目的:以超高效液相质谱联用法鉴定了乔松素在大鼠体内外的代谢产物。方法:分析乔松素经大鼠肝微粒体体外代谢和大鼠口服给药后的胆汁、尿液、粪便中的代谢产物,以超高效液相质谱联用法分离鉴定代谢产物。结果:在大鼠肝微粒体和胆汁、尿液、粪便中共检测到5个代谢产物,根据裂解规律及与标准品对照,鉴定了其中3个代谢产物的结构,推测了另外2个代谢产物的结构。结论:通过大鼠体内外代谢产物的比较,发现乔松素的代谢途径主要是羟基化和葡萄糖醛酸化。其原型和柚皮素是在体内的主要作用形式,在胃肠道及肝脏中的葡萄糖醛酸化是其代谢失活的主要途径。
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| 关 键 词： | 乔松素 代谢产物 UPLC-MS/MS |
UPLC-MS/MS Analysis on Metabolites of Pinocembrin in Vivo and in Vitro |
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| LI Yuan-yuan,ZHAO Ting,XU Chong,WANG Zheng-tao.UPLC-MS/MS Analysis on Metabolites of Pinocembrin in Vivo and in Vitro[J].Chinese Archives of Traditional Chinese Medicine,2012(4):752-755. |
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| Authors: | LI Yuan-yuan ZHAO Ting XU Chong WANG Zheng-tao |
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| Institution: | 2,3(1.Zhejiang Medical College,Hangzhou 310053,Zhejiang,China; 2.The MOE Key Laboratory for Standardization of Chinese Medicines,Institute of Chinese Materia Medica,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China; 3.Shanghai R&D Centre for Standardization of Chinese Medicines,Shanghai 201203,China) |
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| Abstract: | Objective:To characterize the metabolism of pinocembrin in rat liver microsome and in vivo.Methods:The metabolites of pinocembrin in rat liver microsome and in bile,urine,faeces were studied using UPLC-MS/MS method.Result:Five metabolites were detected and identified.Their structures were elucidated on the basis of reference standard and MS/MS spectra.Conclusion:The results indicated that the main metabolic pathways of pinocembrin were hydroxylation and glucuronidation.Pinocembrin and naringenin were the effective components in vivo.Glucuronidation was the main deactivation pathway of pinocembrin. |
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| Keywords: | pinocembrin metabolite UPLC-MS/MS |
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