华蟾素调控VEGF／VEGFR-2信号传导抑制肿瘤血管生成的研究

目的:探讨华蟾素注射液对肿瘤血管生成影响及其调控相关信号传导的分子机制。方法:采用Lewis肺癌荷瘤小鼠随机分为生理盐水组(阴性对照组),参一组(阳性对照组),华蟾素组,观察荷瘤小鼠肿瘤抑制率,瘤内微血管密度;采用免疫组化法检测VEGF表达;采用Western Blotting法检测人脐静脉血管内皮细胞VEGFR-2(KDR)表达。结果:华蟾素的抑瘤率在12%～31%;华蟾素能减少瘤内血管密度、降低瘤内VEGF表达及血管内皮细胞膜上VEGFR-2蛋白表达,华蟾素组与生理盐水组比较有显著差异(P<0.05)。结论:华蟾素能抑制肿瘤血管生成,其作用机制与调控VEGF/VEGFR-2信号传导有关。

Mechanism Investigation of Huachansu in Regulating Signal Transduction of Tumor Angiogenesis

Objective:To investigate the effect of Huachansu(HCS) on angiogenesis and the mechanism of which is regulation in signal transduction. Methods: Mice transplanted with Lewis lung cells were randomly divided into saline group (negative control), Rg3 group (positive control) and HCS group. The tumor inhibition rate and the quantity of microvessel density were observed; The expression of VEGF of implanted tumor of mice was tested by immunohistochemistry . The expression of VEGFR-2 of HUVECs was tested by western blotting method.Results: The results showed the tumor inhibited rates of HCS were 12%~31%. HCS reduced the quantity of microvessel density in tumor and lowered the expression of VEGF of tumor and VEGFR-2 of HUVECs,HCS group was lower than those in the saline group, significant difference was shown in comparison with those in the saline group (P<0.05). Conclusion: HCS could inhibit angiogenesis, the mechanism of which is the regulation of VEGF/ VEGFR-2 pathway.