| HPLC-MS法鉴定广西产拳卷地钱中5种黄酮类成分 |
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| 引用本文： | 朱华,牛思琪,龙莉,黎理,谢凤凤,笪舫芳,秦丽.HPLC-MS法鉴定广西产拳卷地钱中5种黄酮类成分[J].中华中医药学刊,2020(3):17-23. |
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| 作者姓名： | 朱华 牛思琪 龙莉 黎理 谢凤凤 笪舫芳 秦丽 |
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| 作者单位： | 广西中医药大学;广西民族大学 |
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| 基金项目： | 国家自然科学基金(81260620);广西壮瑶药协同创新中心项目(桂教科研[2013]20);广西壮瑶药重点实验室(桂科基字[2014]32);广西重点学科壮药学项目(桂教科研[2013]16);南宁市科技局项目(20183046-1)。 |
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| 摘 要： | 目的采用HPLC-MS鉴定广西产拳卷地钱75%乙醇提取物中黄酮类成分。方法采用色谱柱:ACQUITY UPLC HSS T3 C18(2.1 mm×100 mm,1.8μm),流动相为0.1%甲酸水(A)-乙腈(B),梯度洗脱,质谱使用电喷雾电离源(ESI)正负离子模式。结果分析得出拳卷地钱含有黄酮类成分5个,分别是Apigenin7,4’-diglucuronide、Apigenin7-glucuronide、芹菜素、木犀草素、金圣草黄素。结论该方法稳定、准确性好,可用于广西产拳卷地钱成分鉴定及质量控制。
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| 关 键 词： | HPLC-MS 鉴定 广西 拳卷地钱 黄酮类成分 |
Five Flavonoids in Marchantia convolutain Guangxi Identified by HPLC-MS |
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| ZHU Hua,NIU Siqi,LONG Li,LI Li,XIE Fengfeng,DA Fangfang,QIN li.Five Flavonoids in Marchantia convolutain Guangxi Identified by HPLC-MS[J].Chinese Archives of Traditional Chinese Medicine,2020(3):17-23. |
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| Authors: | ZHU Hua NIU Siqi LONG Li LI Li XIE Fengfeng DA Fangfang QIN li |
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| Institution: | (Guangxi University of Chinese Medicine,Nanning 530200,Guangxi,China;Guangxi University for Nationalities,Nanning 530006,Guangxi,China) |
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| Abstract: | Objective The flavonoids content in 75% alcohol extract was identified by HPLC-MS in Marchantia convoluta in Guangxi. Method Chromatographic column ACQUITY UPLC HSS T3 C18 (2.1 mm×100 mm, 1.8 μm) was adopted. The mobile phase was 0.1% formate water(A)-acetonitrile(B), gradient elution, and mass spectra used an electrospray ionization source(ESI) positive and negative ion mode. Result According to the analysis, there may be 5 flavonoids, namely Apigenin7,4’-diglucuronide, apigenin7-glucuronide, celery, luteolin and chrysophane. Conclusion The method is stable and accurate, and can be used for the identification and quality control of marchantia convoluta in Guangxi. |
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| Keywords: | HPLC-MS identification Guangxi Marchantia convoluta flavonoids |
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