解郁除烦胶囊对抑郁症模型小鼠行为学及脑内单胺类神经递质的影响

目的：观察解郁除烦胶囊对抑郁症模型小鼠行为学及脑内单胺类神经递质的影响，探讨其作用机制。方法将ICR雄性小鼠按体重随机分为模型组，解郁小、中、大剂量组，百优解组，阿米替林组，正常对照组。解郁小、中、大剂量组分别灌胃解郁除烦胶囊混悬液1.250、2.500、5.000 g/kg，百优解组小鼠灌胃百优解混悬液0.014 g/kg，阿米替林组灌胃阿米替林混悬液0.050 g/kg，正常对照组和模型组灌胃等体积生理盐水，1次/d。除正常对照组外，其余各组小鼠采用腹腔注射利血平注射液制备抑郁症小鼠模型。连续给药第22天，观察各组小鼠的眼睑下垂及运动情况；给药第25天，检测小鼠脑组织单胺类神经递质水平变化。结果与模型组比较，注射利血平注射液后1、2、6 h，解郁大剂量组小鼠眼睑闭合只数减少(P＜0.05或P＜0.01)；注射利血平注射液后4 h，解郁中、大剂量组小鼠未出圈只数减少(P＜0.05或 P＜0.01)；解郁中、大剂量组小鼠脑组织中去甲肾上腺素(880.45±428.81)ng/g、(875.98±449.33)ng/g比(299.92±267.08)ng/g]、多巴胺(2305.99±530.37)ng/g、(2169.99±278.19)ng/g比(1439.34±357.33)ng/g]水平升高(P＜0.01)；解郁中剂量组小鼠脑组织中5-羟色胺(781.43±135.10)ng/g比(492.01±192.80)ng/g]水平较模型组升高(P＜0.01)。结论解郁除烦胶囊对利血平抑郁症模型小鼠具有抗抑郁作用，其作用机理可能与调节中枢神经中单胺类神经递质水平去甲肾上腺素、多巴胺、5-羟色胺有关。

Effects of Jieyu-Chufan capsule on the behavior and the level of cerebral monoamine ;neurotransmitter in depression mouse model

Objective To observe the effects of Jieyu-Chufan capsule(JCC) on the behavior and the monoamine neurotransmitter content of cerebral in depression mouse model and explore its mechanism. Methods The ICR male mouse were randomly divided into seven groups according to their weights(the model group, the low, middle and high doses group, Baiyoujie group, the amitriptyline group, the normal control group). The low, middle and high doses group were given JCC with 1.250, 2.500, 5.000 g/kg separately by intragastric administration. Baiyoujie group was given 0.014 g/kg Baiyoujie by gavage. The amitriptyline group was given 0.050 g/kg amitriptyline by gavage. The model group and the normal control group received the same volume of distilled water intragastrically. The drugs were administrated once a day. The brepharoptosis and movement conditions of mouse were observed after 22 days. The monoamine neurotransmitter content of each group was measured after 25 days. Results Compared to the model group, the number of mouse with brepharoptosis decreased in the high dose group after 1, 2, 6 hour with reserpine injection (P<0.05 or P<0.01);the number of mouse came out from the circle increased after 4 hours with reserpine injection (P<0.05 or P<0.01);the content of NA (880.45 ± 428.81 ng/g, 875.98 ± 449.33 ng/g vs. 299.92 ± 267.08 ng/g) and DA (2 305.99 ± 530.37 ng/g, 2 169.99 ± 278.19 ng/g vs. 1 439.34 ± 357.33 ng/g) in the middle and high dose group increased (P<0.01);the content of 5-HT (781.43 ± 135.10 ng/g vs. 492.01 ± 192.80 ng/g) in the middle group increased (P<0.01). Conclusions JCC showed an antidepression influence in depression mouse model induced by reserpine. The mechanism may be related to regulating the level of monoamine neurotransmitter in the nervous centralis .