全蝎蛋白药效组分对L1210肿瘤细胞凋亡的影响

目的 研究全蝎(Scorpio)蛋白药效组分对L1210肿瘤细胞凋亡的影响,为建立全蝎与临床疗效对应的生物效应质量评价方法奠定基础.方法 采用改良MTT法、流式细胞法研究全蝎蛋白药效组分对L1210细胞毒和细胞周期抑制的作用.结果 抑制癌细胞生长的浓度≥37 mg/ml,剂量与浓度呈正比,相关系数为0.9357,IC50为175 mg/ml,显效时间0～48 h;全蝎蛋白药效组分浓度≥9.25 mg/ml时,可显著提高L1210细胞的凋亡率.结论 全蝎蛋白药效组分具有促进L1210细胞凋亡、抑制增殖的作用,其抗肿瘤生物效应指标为:9.25～175 mg/ml,可作为全蝎生物效应质量评价的指标之一.

The inhibitive effects of the protein active composition of Scorpio on apoptosis of L1210 tumor cells

Objective To investigate the effects of protein active composition of Scorpio on apoptosis of L1210 tumor cells for the purpose of establishing the quality evaluation method of biological effect of Scorpio. Methods L1210 cells were examined by trypan blue exclusion. The proliferation of cells was determined by improved MTT assay. Flow cytometry was performed to analyze cell apoptosis with propidium iodide (PI). Results When the concentration of protein active composition of Scorpio exceeded or equaled 37 mg/ml, the coefficient correlation of the growth inhibiting curve of L1210 cells was 0.9357, and IC50 was 175 mg/ml. The excellence time was 0 to 48 hours. When the concentration of protein active composition of Scorpio exceeded or equaled 9.25 mg/ml, the apoptosis ratio of L1210 cells was raised significantly.Conclusion The protein active composition of Scorpio might promote the apoptosis and restrain the proliferation of L1210 cells. The value of anti-tumor biological effect of the protein active composition of Scorpio was 9.25 ～ 175 mg/ml. This value may be one of the indexes for quality evaluation of biological effect of Scorpio.