| 银杏外种皮多糖对HL-60细胞的体外实验研究 |
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| 引用本文： | 许爱华,陈华圣,孙步蟾.银杏外种皮多糖对HL-60细胞的体外实验研究[J].中药材,2004,27(5):361-363. |
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| 作者姓名： | 许爱华 陈华圣 孙步蟾 |
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| 作者单位： | 扬州大学医学院,扬州,225001 |
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| 基金项目： | 江苏省社会发展基金，江苏省教育厅自然科学基金 |
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| 摘 要： | 目的:研究银杏外种皮多糖对体外HL-60细胞的作用.方法:运用MTT方法和流式细胞技术,检测HL-60细胞体外增殖、凋亡以及相关基因的表达.结果:银杏外种皮多糖(40～160 m/L,48h)可抑制HL-60细胞增殖及增殖促进基因c-myc的表达,可诱导HL-60细胞凋亡并下调凋亡抑制基因bcl-2的表达.结论:银杏外种皮多糖对HL-60细胞增殖及凋亡的影响涉及增殖促进基因c-myc和凋亡抑制基因bcl-2.
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| 关 键 词： | 银杏外种皮多糖 HL-60细胞 增殖 凋亡 基因 |
| 修稿时间： | 2003年10月15 |
Experimental Study of Ginkgo biloba Exocarp Polysaccharides on HL-60 Cells in Vitro |
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| Xu Aihua,Chen Huasheng,Sun Buchan.Experimental Study of Ginkgo biloba Exocarp Polysaccharides on HL-60 Cells in Vitro[J].Jorunal of Chinese Medicinal Materials,2004,27(5):361-363. |
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| Authors: | Xu Aihua Chen Huasheng Sun Buchan |
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| Institution: | Medical College of Yangzhou University, Yangzhou 225001. |
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| Abstract: | OBJECTIVE: To study the effect of Ginkgo biloba exocarp polysaccharides (GBEP) on HL-60 cells in vitro. METHOD: Cell culture, MTT, flow cytometry were performed to observe proliferation, apoptosis and changes of relevant gene expression of HL-60 cells. RESULT: GBEP (40-160 mg/L, 48 h) could inhibit HL-60 cells proliferation and the expression of proliferation-promotion gene c-myc, induce HL-60 cells apoptosis and down-regulate the expression of apoptosis-inhibitory gene bcl-2. CONCLUSION: Influence of GBEP on HL-60 cells proliferation and apoptosis may relate to its effects on the expression of proliferation-promotion gene c-myc and apoptosis-inhibitory gene bcl-2. |
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| Keywords: | GBEP HL-60 cell Proliferation Apoptosis Gene |
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