黄精不同炮制品中5-羟甲基糠醛的含量测定

目的通过对不同炮制时间5-羟甲基糠醛含量变化规律的考察,为黄精的炮制工艺及质量标准研究奠定基础.方法超声提取法获得供试品溶液,HPLC法测定其含量;色谱条件分析柱Agilent HC-C18柱(5 μm,250 mm×4.6 mm),甲醇-水(1585)为流动相,流速为1 ml/min,检测波长为284 nm.结果黄精不同炮制品中都检测出5-羟甲基糠醛,蒸制30 h内含量基本稳定,但受热30 h以后含量急剧上升,继续加热含量下降.结论该结果能够为黄精质量控制和饮片加工与规范化炮制提供参考数据.

Determination of 5-Hydroxymethyl Furfuraldehyde in Different Processed Products of Polygonatum

OBJECTIVE: By finding the changing regularity of 5-Hydroxymethyl furfuraldehyde (5-HMF) content in Polygonatum for different time, this provides basis for the technology of processing Polygonatum and quality standards of its prepared pieces. METHODS: The testing solution was acquired by ultrasonic extraction, HPLC was used to determine the contents. The chromatographic conditions: Agilent HC-C18 column (5 microm, 250 mm x 4.6 mm). The mobile phase: methanol-water (15:85) at the flow rate of 1 ml/min. The determination wave length was 284 nm. RESULTS: All kinds of processed Polygonatum had 5-Hydroxymethyl furfuraldethyde in different degrees, contents in Polygonatum kept relatively stable before 30 hours, but contents rose rapidly after 30 hours, contents decreased as continuing heating. CONCLUSION: The result can provide useful references to quality control and standard porcessing condition of Polygonatum.