大孔吸附树脂纯化丹参水溶性有效成分的研究

目的:优选大孔吸附树脂纯化丹参水溶性有效成分的工艺条件。方法:采用高效液相色谱法,以丹酚酸B的含量为指标,对大孔吸附树脂型号、吸附条件、洗脱条件进行了考察。结果:最佳工艺条件为采用XDA-5型大孔吸附树脂,控制药液浓度以丹酚酸B计约为18 mg/ml,pH=4.0,吸附过柱,以70%乙醇3倍柱体积洗脱,丹酚酸B洗脱率在90%以上。结论:优选工艺可较好的纯化富集丹参水溶性有效成分。

Optimization of Preparative Separation and Purification of Water-soluble Substance from Salvia miltiorrhiza by Macroporous Resins

Objective: To study the optimum conditions for the isolation and purification of water-soluble substance from Salvia miltiorrhiza Bge..Methods: The optimum macroporous resin was selected and the separation and purification process was evaluated by measuring the content of Salvianolic acid B in the fractions by HPLC.Results: The XDA-5 macroporous resin was the most effective compared with other macroporous resins.The optimum conditions were screened,which were 18 mg/ml corresponding to Salvianolic acid B for concentration of extract,pH was 4,and the volume of 70%(V/V) ethanol as eluant was 3 BV.By this method,the elution efficiency of Salvianolic acid B exceeded 90%.Conclusion: The method is more effective for large-scale isolation and purification of water-soluble substance from Salvia miltiorrhiza Bge..