积雪草苷对LPS诱导小鼠急性肺损伤炎症因子平衡的影响

目的：观察积雪草苷对脂多糖（LPS）诱导急性肺损伤支气管肺泡灌洗液（BALF）IL-6、TNF-α、IL-10的影响。方法：56只Balb/c小鼠随机分为空白、模型、假手术、溶媒、积雪草苷5,15,45mg/kg剂量组。LPS（2.5mg/kg）气管滴注造急性肺损伤模型,24h后处死小鼠,酶联免疫吸附法（ELISA）检测血浆IL-6、TNF-α、IL-10表达。结果：积雪草苷各剂量组呈剂量依赖性降低LPS所致急性肺损伤模型BALF中IL-6、TNF-α含量,增加IL-10表达。结论：积雪草苷对LPS诱导急性肺损伤保护作用可能与抑制炎症因子IL-6、TNF-α和促进IL-10,维持炎症因子间平衡有关。

Effects of Asiaticoside on the Balance of Inflammatory Factors of Mouse's Acute Lung Injury Induced by LPS

OBJECTIVE: To observe the effect of asiaticoside (AS) on IL-6,TNF-alpha, IL-10 of bronchoaliveolar lavage fluid (BALF) of acute lung injury (ALI) induced by lipopolysaccharides (LPS). METHODS: 56 Balb/c mice were randomly divided into control, model, sham operation,vehicle, model + asiaticoside 5, 15, 45 mg/kg groups. Model of ALI were established by intratracheal instillation of LPS 20 microl ( 2.5 mg/kg), mice were killed 24 hours later, the content of IL-6 ,TNF-alpha, IL-10 of BALF was detected by enzyme-linked immunosorbentassay (ELISA). RESULTS: AS could reduce the content of IL-6, TNF-alpha and increase IL-10 of BALF in dose-dependent manner. CONCLUSION: The protective effects against ALL induced by LPS on AS are related to reducing the content of IL-6, TNF-alpha, increasing secretion IL-10 and keeping the balance between inflammatory factors and anti-inflammatory factors.