| 基于rDNA-ITS序列及相似度分析的中药赤芍分子鉴别研究 |
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| 作者姓名： | Liu J Liu CS Wei SL Zhang J Zheng J |
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| 作者单位： | 北京中医药大学中药学院;中国药品生物制品检定所; |
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| 基金项目： | 重大新药创制(2009ZX9308-001-4) |
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| 摘 要： | 目的:分析中药赤芍正品及混伪品ITS序列的种内相似度及特定位点序列碱基差异,为建立中药赤芍的分子鉴别标准提供思路。方法:采用PCR扩增产物直接测序的方法对市场上购得的未知来源赤芍样品S1、S2的ITS序列(包括ITS1,ITS2,5.8S)进行测定,测定结果进行BLAST比对、相似度分析、序列特异性碱基位点差异比较;用DNAMAN对GenBank上已注册的正品赤芍和混伪品赤芍的ITS序列进行分析。结果:建立了赤芍药材分子鉴别的3个指标,即根据BLAST结果定属,种内相似度缩小属内范围并定种,再通过特异性碱基位点差异最终确定并证实样品的物种;确定了样品S1来源于芍药属植物芍药Paeonia lactiflora Pall.,样品S2来源于芍药属植物川赤芍Paeonia veitchii Lynch.。结论:本文为赤芍的分子鉴别提供较为有效的思路与方法,同时为基于rDNA-ITS相似度及序列分析进行其他中药品种鉴别提供依据。
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| 关 键 词： | 赤芍 ITS序列 相似度 序列分析 |
Analysis of rDNA-ITS sequences and similarity of radix paeoniae |
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| Liu J,Liu CS,Wei SL,Zhang J,Zheng J.Analysis of rDNA-ITS sequences and similarity of radix paeoniae[J].Jorunal of Chinese Medicinal Materials,2011,34(10):1517-1521. |
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| Authors: | Liu Juan Liu Chun-Sheng Wei Sheng-Li Zhang Ji Zheng Jian |
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| Institution: | LIU Juan1,LIU Chun-sheng1,WEI Sheng-li1,ZHANG Ji2,ZHENG Jian2(1.School of Chinese Materia Medica,Beijing University of Traditional Chinese Medicine,Beijing 100102,China,2.National Institutes for Food and Drug Control,Beijing 100050,China) |
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| Abstract: | Objective: To establish molecular authentication standards of Radix Raeoniae.Methods: PCR method was used to determine rDNA-ITS sequences of sample S1 and sample S2 including ITS1,5.8S,and ITS2.The sequences were analyzed by BLAST and DNAMAN software.rDNA-ITS sequences of Paeonia lactiflora Pall.,Paeonia veitchii Lynch.and their counterfeit were analyzed by DNAMAN software.Results: Established three molecular authentication standards on Radix Paeoniae:(1)The genera of samples was determined by the results o... |
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| Keywords: | Radix Paeoniae ITS sequence Similarity Sequence analysis |
| 本文献已被 CNKI PubMed 等数据库收录！ |
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