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泽漆根体外抗肿瘤实验研究
引用本文:蔡鹰,王晶.泽漆根体外抗肿瘤实验研究[J].中药材,1999,22(2):85-87.
作者姓名:蔡鹰  王晶
作者单位:解放军第454医院,解放军第454医院,解放军第454医院 南京 210002,南京 210002,南京 210002
摘    要:本文研究了泽漆根水提取液(EWE)的体外抗肿瘤作用。通过活细胞计数法、MTT法、集落形成试验观察其对肿瘤细胞的生长抑制作用。活细胞计数结果表明EWE对7721、Hela、MKN-45细胞株72小时IC_(50)分别为1.26、1.98、1.72mg/ml;EWE对三种细胞降解MTTIC_(50)值分别为 1.43、1.67、0.97mg/ml;EWE4mg/ml对三种细胞集落抑制率分别为59.8%、66.4%、70.5%。说明EWE有直接细胞毒作用,并与时间、剂量呈正相关。

关 键 词:泽漆根  人肝癌7721细胞株  人宫颈癌Hela细胞株  人胃癌MKN-45细胞株  体外抗肿瘤试验

Antitumor Activity of the Root of Euphorbia helioscopic in Vitro
Cai Ying,Wang Jing,Liang Bingwen.Antitumor Activity of the Root of Euphorbia helioscopic in Vitro[J].Jorunal of Chinese Medicinal Materials,1999,22(2):85-87.
Authors:Cai Ying  Wang Jing  Liang Bingwen
Institution:No. 454 Hospital of PLA, Nangjing 210002.
Abstract:Antitumor activity of the aquatic extract the root of Euphorbia helioscopia L (EWE)in Vitro were studied. Viable cells count, MTT staining and colonal formation assays of three kinds of cancer cells were used to assess the antitunior activity. Determined by viable cells count, the IC50 values of EWE against 7721, Hela, MKN-45 cells were 1.26, 1.98, 1.72mg/ml respectively (72h). Determined MTT staining, the IC50 values of EWE against 7721, Hela, MKN-45 cells were 1.43, 1.67, 0.97mg/ml. Determined by colonal formation, the inhibition rate of EWE (4mg/ml) against 7721, Hela, MKN-45 cells were 59.8%, 66.4%, 70.5%. The results indicated that EWE had obvious antitumor activity.
Keywords:The roots of Euphorbia helioscopia  Antitumor  7721 cells  Hela cells  MKN-45 cells  
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