加味四逆散对束缚制动心理应激大鼠HPA轴调节作用的影响

目的观察加味四逆散对束缚制动所致慢性心理应激大鼠HPA轴调节作用的影响及其胸腺免疫抑制的途径.方法大鼠随机分为正常对照组(C组)、模型组(M组)、加味四逆散组(C1组)、人参皂苷组(C2组).采用放射免疫方法检测大鼠下丘脑CRH、血浆ACTH、CORT及胸腺细胞糖皮质激素受体的数目与核转移率.结果与C组相比,M组下丘脑CRH、血浆ACTH、CORT显著增高(P＜0.01),而C1、C2组大鼠下丘脑CRH、血浆ACTH与CORT含量显著降低(P＜0.05或P＜0.01).M组胸腺糖皮质激素受体的核转移率显著高于C组(P＜0.01),而C1、C2组的核转移率均显著低于M组,且C1组各项检测数据与C组比较无统计学差异.结论加味四逆散有效参与了心理应激大鼠HPA轴的调节,并显著减轻糖皮质激素对胸腺的抑制作用,其作用途径与HPA轴糖皮质激素受体的介导密切相关.

Effect of Jiawei Sini Decoction on HPA Function of Rats with Restraint Psychological Stress

OBJECTIVE: To observe the effect of Jiawei Sini Decoction (JWSND) on HPA function of rats with restraint psychological stress and the pathway of thymus immunosuppressive function. METHODS: The rats were randomly divided into 4 groups, control group (C), model group (M), group treated by JWSND ( C1) and ginsenosides ( C2). Hypothalamus CRH, blood plasma ACTH and CORT, the numberofthy moeyte GCR sites and the GCR nuclear translocation rate were detected by radioimmunoassay. RESULTS: Compared with C, in M, Hypothalamus CRH, blood plasma ACTH and CORT increased significantly (P < 0.01), but all these of C1 and C2 lowered significantly (P < 0.05, P < 0.01). Compared with C, the GCR nuclear translocation rate was increased significantly(P < 0. 01). Compared with M, the GCR nuclear translocation rate lowered significantly ( P < 0.01). Moreover, no significant difference was found in all indexes between C1 and C. CONCLUSION: JWSND participates effectively in the adjustment of HPA of the rats with restraint psychological stress, and relieves significantly the inhibitory effect of glucocorticoid on the thymus, the pathway closely relates to the translocation of GCR.