肿节风提取物对鼻咽癌裸鼠移植瘤细胞凋亡的影响

目的:探讨肿节风提取物对人鼻咽癌细胞株CNE1、CNE2裸鼠移植瘤的抑瘤作用及与瘤细胞凋亡的关系。方法:采用CNE1、CNE2细胞接种于裸鼠腋下,1周后成瘤,建立肿瘤模型,灌胃给药30 d;TUNEL法检测细胞凋亡;流式细胞仪进行细胞凋亡的分析;透射电镜观察瘤组织细胞超微结构的改变;免疫组化法检测瘤组织Bc l-2,Bax的表达。结果:肿节风治疗组的裸鼠瘤重、肿瘤相对体积明显低于对照组(P<0.01);TUNEL法检测肿节风组凋亡指数高于对照组(P<0.01);流式细胞技术显示肿节风组凋亡率分别为58.34%和63.76%,与对照组相比有统计学意义(P<0.01);电镜下用药组瘤组织中可见较多典型的细胞凋亡的形态学表现;免疫组化结果显示Bc l-2蛋白的表达明显低于对照组(P<0.01),而Bax蛋白的表达明显高于对照组(P<0.01)。结论:肿节风在体内具有抑制CNE1、CNE2移植瘤生长的作用,其机制与下调Bc l-2蛋白、上调Bax蛋白的表达进而促进肿瘤细胞的凋亡有关。

Study on the Apoptosis of Nasopharyngeal Carcinoma Cell Line Administrated with Sarcandra glabra Extracts in vivo and its Mechanism

Objective: To evaluate the effect of Sarcandra glabra extracts on anti-tumor and apoptosis of implanted human nasopharyngeal carcinoma cell lines in nude mice.Methods: Models of CNE1 and CNE2 xenograft in nude mice were established to investigate the anti-tumor effect of Sarcandra glabra.The apoptosis of CNE1 and CNE2 cells were observed with TUNEL and FACS with PI-staining.The ultra micro-structured changes of CNE1 and CNE2 cells were observed by transmission electron microscope.The expression of Bcl-2 and Bax of xenografts were investigated by immunohistochemistry.Results: The Sarcandra glabra extracts significantly inhibited the growth of tumor when compared with normal control group.The expression of Bcl-2 was lower(P <0.01) and the expression of Bax of the Sarcandra glabra group was higher(P <0.01) than that of normal control group.The rate of cell apoptosis exhibiting was higher than that of normal control group(P <0.01).Electron microscopy indicated the apoptosis of tumor cells with marginal nuclei,chromatin condensation and nuclei fragmentation,and apoptotic bodies were observed.Conclusion: Sarcandra glabra suppresses the growth of tumor in vivo.The mechanism is associated with down-regulating the expression of Bcl-2 and up-regulating the expression of Bax to promote apoptosis.