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三七及其伪品的DNA测序鉴别
引用本文:曹晖,刘玉萍,伏见裕利,小松かっ子.三七及其伪品的DNA测序鉴别[J].中药材,2001,24(6):398-402.
作者姓名:曹晖  刘玉萍  伏见裕利  小松かっ子
作者单位:1. 中国中医研究院中药研究所
2. 富山医科药科大学和汉药研究所药效解析中心
摘    要:目的:分析三七Panax natoginseng及其伪品竹节参P.japoricuscus、蓬莪术Curcuma phaeocaulis、温莪术C.wenyujin、桂莪术C.kwangsiensis的核基因和叶绿体基因序列,为三七的正品药材基原鉴定提供分子依据。方法:采用PC8直接测序技术测定三七及其4种伪品的18S rRNA基因和matK基因核苷酸序列并作序列变异分析。结果:三七和竹节参的18S rRNA基因序列长度相同,均为1809bp;matK基因长度亦相同,为1259bp。蓬莪术、温莪术和桂莪术18S rRNA基因长度均为1811bp、marK均为1548bp。根据排序比较,三七与4种伪品间的DNA序列存在很大差别。结论:通过序列差异比较分析,DNA测序技术可成为三七正品基原鉴定的准确、有效手段。

关 键 词:三七  莪术  伪品  matK基因  基原  rRNA基因  正品  竹节参  核基因  技术测定
修稿时间:2001年1月3日

Identification of Notoginseng ( Panax notoginseng) and Its Adulterants Using DNA Sequencing
Cao Hui,Liu Yuping,Fushimi,Komatsu K.Identification of Notoginseng ( Panax notoginseng) and Its Adulterants Using DNA Sequencing[J].Jorunal of Chinese Medicinal Materials,2001,24(6):398-402.
Authors:Cao Hui  Liu Yuping  Fushimi  Komatsu K
Institution:Institute of Chinese Materia Medica, China Academy of Traditional Chinese Medicine, Beijing 100700.
Abstract:Objective: To analyze the nuclear ribososmal RNA small subunit (18S rRNA) and chloroplast matK gene sequence of notogin-seng ( Panax notoginseng) in order to provide molecular evidence for its genuine origin identification. Methods: To sequence 18S rRNA and matK genes of Panax notoginseng and its four adulterants such as P. japonicus , Curcuma phaeocaulis , C. wenyujin, C. kwangsiensis using PCR direct sequencing and to detect their variation of sequences. Results: The sequence length of notoginseng and its adulterants is 1809-1811 bp for 18S rRNA gene and 1259-1548 bp for matK gene, respectively. Multiple sequence alignment shows that there are much sequence variation between notoginseng and its adulterants. Conclusion: DNA sequencing is an accurate and reliable method in origin identification of the genuine notoginseng.
Keywords:Notoginseng  Panax notoginseng(Burk  ) F  H  Chen  18S rRNA gene  matK gene  DNA sequencing  Identification of traditional Chinese medicine
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