| 瑞香狼毒系统溶剂法分离提取部位的体外抗肿瘤活性 |
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| 引用本文： | 王彬,王锐,贾正平,樊俊杰,马骏,张汝学.瑞香狼毒系统溶剂法分离提取部位的体外抗肿瘤活性[J].中药材,2004,27(5):355-357. |
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| 作者姓名： | 王彬 王锐 贾正平 樊俊杰 马骏 张汝学 |
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| 作者单位： | 1. 兰州大学生命科学学院,兰州,730000
2. 兰州军区兰州总医院药局,兰州,730050 |
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| 摘 要： | 目的:分离筛选瑞香狼毒中抗肿瘤活性部位.方法:用系统溶剂法对瑞香狼毒95%乙醇提取物进行分离,MTT比色法对分离得到的部位进行体外抗肿瘤活性的测定.结果:石油醚提取物和氯仿提取物对体外培养肿瘤细胞表现出较强的抑制作用,在测定浓度范围内呈现出良好剂量依赖性抑制作用,而正丁醇和甲醇提取部位在测定浓度范围内则显示出很弱的抑制作用;在各提取部位中石油醚提取物的体外抗肿瘤作用最强.结论:石油醚提取部位是瑞香狼毒中的体外抗肿瘤活性部位.
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| 关 键 词： | 瑞香狼毒 系统溶剂法 抗肿瘤活性 |
| 修稿时间： | 2003年9月8日 |
The Antitumor Activities of the Extracts from Stellera chamaejasmel L.in Vitro by Means of Systematic Solvent Extraction |
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| Wang Bin,Wang Rui,Jia Zhengping,Fan Junjie,Ma Jun,Zhang Ruxue.The Antitumor Activities of the Extracts from Stellera chamaejasmel L.in Vitro by Means of Systematic Solvent Extraction[J].Jorunal of Chinese Medicinal Materials,2004,27(5):355-357. |
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| Authors: | Wang Bin Wang Rui Jia Zhengping Fan Junjie Ma Jun Zhang Ruxue |
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| Institution: | School of Life Science, Lanzhou University, Lanzhou 730000. |
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| Abstract: | Objective:To screen the antitumor extract from Stellera chamaejasmel L.. Methods:The means of systematic solvent extraction was used to separate the 95% ethanol extract. The antitumor activities of the different extracts in vitro were analyzed with MTT assay. Results:The petroleum ether extract and the chloroform extract strongly inhibited cell proliferation of human cancer cell lines such as leukemia HL-60, stomach cancer SGC-7901 and hepatocarcinoma BEL-7402,and showed significant dose-dependent response while the n-butanol extract and the methanol extract exhibited low antitumor activities in vitro. The petroleum ether extract was most active. Conclusion:The petroleum ether extract is the principal antitumor extract from Stellera chamaeiasmel L.. |
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| Keywords: | MTT |
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