基于生物热活性表达的中药板蓝根品质评价研究

目的：建立基于生物热活性表达的中药板蓝根品质评价研究模式和方法。方法：分别采用常规化学方法和生物热动力学方法分析不同板蓝根的化学差异及作用于细菌、病毒和免疫细胞的生物热活性差异,比较化学方法和生物方法表达板蓝根品质的异同,并应用计算机模式识别技术建立品质评价数学模型。结果：板蓝根中有机酸、多糖含量与品质有一定的相关性,但含量＋化学指纹图谱的方法难以准确区分不同品质的板蓝根样品;而基于4个主要生物热活参数建立的数学模型可以较好的判断板蓝根的品质,误判率仅为1.39%。结论：本文建立的基于生物热活性表达的板蓝根品质评价模型较常规化学方法更准确、可靠。

Study on Quality Evaluation of Radix Isatis Based on Analysis of Biothermic Activity

OBJECTIVE: To establish a novel pattern and method to evaluate the quality of Radix Isatis based on analysis of biothermic activity. METHODS: Chemical and biothermodynamic methods were used and compared to investigate the quality of different Radix Isatis samples. And a mathematic model was established by computer aided pattern recognition to evaluate the quality of Radix Isatis. RESULTS: The quality of Radix Isatis was partially related to the content of organic acids and polycose, but it could not be correctly recognized by both chemical determination and HPLC fingerprint. On the other hand, the mathematic model based on the main four parameters of biothermodynamic analysis was very correct (misjudgment ratio of 1.39%) to recognize the quality of Radix Isatis. CONCLUSION: The established model in this paper based on analysis of biothermic activity is more accurate and reliable than that of chemical methods to evaluate the quality of Radix Isatis.