| 远志皂苷对H2O2诱导的PC12细胞损伤的保护作用 |
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| 引用本文： | 孙桂波,邓响潮,李楚华.远志皂苷对H2O2诱导的PC12细胞损伤的保护作用[J].中药材,2007,30(8):991-993. |
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| 作者姓名： | 孙桂波 邓响潮 李楚华 |
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| 作者单位： | 1. 华南师范大学生命科学学院,广东广州,510631
2. 广州中医药大学,广东广州,510405 |
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| 基金项目： | 广东省植物发育工程重点实验室开放基金 |
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| 摘 要： | 目的：观察远志皂苷（Tenuigenin TEN）对过氧化氢（H202）所致PCI2细胞损伤的保护作用。方法：以H2O2损伤PCI2细胞为氧化应激损伤模型，MTT法检测细胞存活率；采用紫外分光光度法测定LDH漏出量，观察细胞的损伤程度；硫代巴比妥酸法测定MDA含量，黄嘌呤氧化酶法测定SOD活性。结果：TEN能明显改善H：0：导致的细胞损伤，与H2O2处理组相比，20、10mg／L组可使细胞存活率升高，LDH漏出量明显降低，降低MDA含量，提高SOD活性。结论：TEN对H2O2诱导的PCI2细胞损伤具有明显的保护作用。
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| 关 键 词： | 远志皂苷 细胞损伤 阿尔茨海默病 |
| 文章编号： | 1001-4454（2007）08-0991-03 |
| 修稿时间： | 2007-04-04 |
The Protective Effects of Tenuigenin on the PC12 Cells Injury Induced by H2O2 |
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| SUN Gui-bo,Deng Xiang-chao,LI Chu-hua.The Protective Effects of Tenuigenin on the PC12 Cells Injury Induced by H2O2[J].Jorunal of Chinese Medicinal Materials,2007,30(8):991-993. |
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| Authors: | SUN Gui-bo Deng Xiang-chao LI Chu-hua |
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| Institution: | 1. College of Life Science,South China Normal University, Guangzhou 510631, China; 2. Editorial Department of Traditional Chinese Drugs Research and Clinical Pharmacology,Guangzhou 510405, China |
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| Abstract: | OBJECTIVE: To study the protective effects of tenuigenin (TEN) on the PC12 cells injury induced by H2O2. METHODS: Oxidative damage PC12 cells model was induced by H2O2. The survival rate of the cells was determined by MTT method. The content of LDH was determined by ultraviolet spectrophometry and the content of MDA and SOD activity was measured respectively by thiobarbituric acid and xanthine oxidese method. RESULTS: tenuigenin could reduce obviously cells injury induced by H2O2. 10 mg L(-1) tenuigenin could improve the cells survival rate, reduce LDH releasing and MDA content, increase SOD activity. CONCLUSION: tenuigenin have significantly potective effect on the PC12 cells injury induced by H2O2. |
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| Keywords: | H2O2 PC12 |
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