植物激素对丹参悬浮培养细胞生长和酚酸类化合物含量的影响

本文报道了２，４－Ｄ、ＢＡ和ＧＡ３对丹参悬浮培养细胞生长和酚酸类化合物含量的影响。在继代培养基（ＭＳ＋２，４－Ｄ１ｍｇ／Ｌ＋ＫＴ０．１ｍｇ／Ｌ）中，培养第１２天细胞生长和迷迭香酸含量达到最大值，而紫草酸Ｂ的含量在培养的第１６天达到最大值，证实产物的形成属于生长依赖型。在相同浓度（１ｍｇ／Ｌ），２，４－Ｄ促进细胞生长和抑制紫草酸Ｂ的形成，对迷迭香酸作用不明显；ＧＡ３抑制细胞生长和促进二种化合物的形成

Effects of Phytohormones on Growth and Content of Depsides in Salvia miltiorrhiza Suspension Cells

This paper deals with the effects of 2,4-D, BA and GA3 on the growth and content of two depsides (rosmarinic acid and lithospermic acid B) in suspension cells of Salvia miltiorrhiza. The results showed cell growth and rosmarinic acid content reached the maximum on the 12th day and lithospermic acid B content on the 16th day after incubating cells in the subculture medium MS + 2, 4-D 1 mg/L + KT 0.1 mg/L. This cell line was a growth-product-associated. With the same concentration (1 mg/L), 2, 4-D stimulated the cell growth but prohibited the formation of lithospermic acid B; GA3 inhabited the cell growth but stimulated the formation of two depsides; The effects of BA is between 2, 4-D and GA3. The concentration optimum of phytohormones tested displayed 3 mg/L for BA and 1 mg/L for GA3. For the optimum time of adding BA and GA3 was in med-term (the 8th day) and early term (at the beginning) of culture period respectively. The synergiatic function of BA and GA3 on the depside formation was also showed that adding GA3 (1 mg/L) was favour for the formation of lithospermic acid B of the suspension cell cultured in the medium containing BA 3 mg/L. The suitable time of adding GA3 was the 6the day after incubating cells in the medium of MS + BA 3 mg/L.