| HPD100大孔吸附树脂在白芍纯化工艺中的应用研究 |
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| 引用本文： | 张亚军,徐莲英.HPD100大孔吸附树脂在白芍纯化工艺中的应用研究[J].中药材,2006,29(9):968-970. |
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| 作者姓名： | 张亚军 徐莲英 |
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| 作者单位： | 1. 上海中医药大学中药学院,上海,201203;西北大学,陕西,西安,710069
2. 上海中医药大学中药学院,上海,201203 |
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| 摘 要： | 目的:研究HPD100大孔吸附树脂纯化白芍提取液的工艺条件及参数。方法:以芍药苷的吸附量和洗脱率为指标,通过单因素试验、正交试验及方差分析,考察HPD100大孔树脂的吸附性能和洗脱参数。结果:HPD100型树脂对芍药苷有良好吸附分离性能,适宜的工艺条件为:上样浓度为0.5 g/m l,最大上样量为10 g/g药材树脂,吸附流速为2 BV/h,洗脱剂为30%乙醇,洗脱剂用量为4倍量吸柱体积。结论:HPD100型大孔吸附树脂在所确定的工艺条件下,对白芍提取液的纯化效果良好,芍药苷纯度可达30%以上。
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| 关 键 词： | HPD100大孔吸附树脂 白芍 芍药苷 纯化 |
| 文章编号： | 1001-4454(2006)09-0968-03 |
| 收稿时间： | 2006-02-07 |
| 修稿时间： | 2006年2月7日 |
Study on Purification of Radix Paeoniae Alba by HPD100 Macroporous Resin |
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| ZHANG Ya-jun,XU Lian-ying.Study on Purification of Radix Paeoniae Alba by HPD100 Macroporous Resin[J].Jorunal of Chinese Medicinal Materials,2006,29(9):968-970. |
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| Authors: | ZHANG Ya-jun XU Lian-ying |
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| Institution: | Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China. |
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| Abstract: | Objective: To set up the method for purification of paeoniflorin by HPD100 macroporous resin.Methods: With the adsorption ration and desorption rate of paeoniflorin as the evaluating quotas,the single factor test,orhogonal experimental designs and variance analysis were applied to optimize the manipulation parameters of macrporous resin.Results: The HPD100 had the best adsorbive and separative properties to paeoniflorin.The applicable technology was as follows: the sample concentration was 0.5 g/ml,the maximum capacity for medicinal materials was 10 g/g,the current velocit was 2 BV/h,the eluting reagent was 30% ethanol(4 times the volume of bottle).Conclusion: HPD100 resin can be used in purify of Radix Paeoniae Alba successfully.The purity of paeoniflorin was above 30%. |
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| Keywords: | Radix Paeoniae Alba Paeoniflorin HPD100 Macroporous resin Purification |
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