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| HPLC-ELSD法测定青蒿中青蒿素的含量 | |
| 引用本文： | 周翱翱,郑文欣,葛发欢.HPLC-ELSD法测定青蒿中青蒿素的含量[J].中药材,2006,29(3):242-245. |
| 作者姓名： | 周翱翱 郑文欣 葛发欢 |
| 作者单位： | 1. 中药提取分离过程现代化国家工程研究中心,广东,广州,510240;中山大学生命科学院,广东,广州,510275 2. 暨南大学,广东,广州,510632 3. 中药提取分离过程现代化国家工程研究中心,广东,广州,510240 |
| 摘 要： | 目的:首次建立高效液相色谱-蒸发光散射检测器测定青蒿素含量的方法,并对大量的青蒿样品进行含量测定。方法:采用蒸发光散射检测器(ELSD)对青蒿药材中的青蒿素进行HPLC分析,色谱柱:迪马公司D iamon-sil C18(250×4.6 mm,5μm);流动相:甲醇-水(75∶25);流速:1 m l/m in;蒸发光散射检测器漂移管温度40℃,载气压力3.5 bar,放大系数(gain)为9;进样体积20μ。l结果:青蒿素在1~5μg范围线性关系良好,回收率为99.33%(RSD=1.97%)。结论:本法具有良好的精密度和重现性,结果准确可靠,可作为青蒿药材及青蒿素类产品的质量控制方法。不同产地、不同采收期青蒿药材中青蒿素的含量测定为青蒿药材的收购及栽培等提供依据。 |
| 关 键 词： | 黄花蒿 青蒿素 HPLC-ELSD法 含量测定 |
| 文章编号： | 1001-4454(2006)03-0242-04 |
| 收稿时间： | 2005-09-15 |
| 修稿时间： | 2005年9月15日 |
Determination of Artemisinin in Artemisia annuna by HPLC-ELSD |
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| ZHOU Ao-ao,ZHENG Wen-xin,GE Fa-huan.Determination of Artemisinin in Artemisia annuna by HPLC-ELSD[J].Jorunal of Chinese Medicinal Materials,2006,29(3):242-245. | |
| Authors: | ZHOU Ao-ao ZHENG Wen-xin GE Fa-huan |
| Institution: | National Engineering Research Center for Modernization of Extraction and Separation Process of TCM, Guangzhou 510240, China. |
| Abstract: | OBJECTIVE: A HPLC method was first established to determine artemisinin in Artemisia annuna by HPLC-ELSD. Artemisinin in Herba of Artemisia annuna from different places was determined by this new method. METHODS: The method utilized MeOH-H2O (75: 25) as mobile phase with flow rate of 1 ml/min, C18 (250 x 4.6 mm, 5 microm) column. The temperature of detector was 40 degrees C, the pressure of N2 was 3.5 bar, and gain value was 9. RESULTS: The calibration curves were linear within the range of 1 - 5 microg, and the average recovery was 99.33% (RSD = 1.97%). CONCLUSION: The method was rapid, convenient, and accurate. It can be used for the quality control of this herbal medicine. |
| Keywords: | Artemisini annua L Artemisinin HPLC-ELSD Content determination |
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