大孔树脂纯化绵茵陈中总有机酸和绿原酸的研究

目的:研究大孔树脂分离纯化绵茵陈中总有机酸和绿原酸的最佳工艺条件。方法:通过对绵茵陈总有机酸和绿原酸在9种大孔树脂上的吸附与解吸附特性的比较,筛选最佳树脂;采用正交试验及单因素考察等方法对该树脂纯化绵茵陈提取液中总有机酸和绿原酸的工艺条件进行优选。结果:以HPD200A型大孔树脂为分离载体,树脂柱径高比为1∶6,样品液浓度为1 g/mL,上柱体积流量为1 BV/h(1 BV=1柱体积),吸附量为生药1.5 g/g树脂,先用3 BV水洗脱除杂,再用2 BV 90%乙醇解吸,过柱后总有机酸和绿原酸的精制度分别为588.74%和567.89%。结论:HPD200A型大孔树脂纯化绵茵陈中总有机酸和绿原酸的工艺简便可靠,可用于规模化生产。

Study on purification of total organic acid and chlorogenic acid in herba artemisiae scopariae with macroporous adsorption resin

Objective:To optimize the technological parameters of the purification process for total organic acid and chlorogenic acid in Herba Artemisiae scopariae with macroporous adsorption resin.Methods:Static and dynamic adsorption-desorption methods were adopted to choose the optimal type of resin.Then orthogonal design L9(34) and single factor experiment were used to select the optimum purification process conditions.Results:The optimal purification process for total organic acid and chlorogenic acid with HPD200A macroporous adsorption resin were as follows:the diameter height ratio of the resin was 1∶6,the sample concentration was 1 g/mL,the absorption velocity was 1 BV/h(1 BV represented one column volume),the ratio of sample to HPD200A macroporous adsorption resin was 1.5∶1(W/W),3 BV of water was used as purificant and 2 BV of 90% ethanol was used as eluant.Under these conditions,the purity of total organic acid and chlorogenic acid was 588.74% and 567.89%,respectively.Conclusion:The established purification process for total organic acid and chlorogenic acid in Herba Artemisiae scopariae with HPD200A macroporous adsorption resin is simple and stable and can be used in industrial production.