| 甘草中-葡α萄糖苷酶抑制剂的初步研究 |
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| 引用本文： | 许有瑞,倪京满,孟庆刚,高燕,范彬.甘草中-葡α萄糖苷酶抑制剂的初步研究[J].中药材,2005,28(10):890-891. |
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| 作者姓名： | 许有瑞 倪京满 孟庆刚 高燕 范彬 |
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| 作者单位： | [1]兰州大学药学院,甘肃兰州730000 [2]金川公司医院药剂科,甘肃金昌737100 [3]甘肃省肿瘤医院药学部,甘肃兰州730050 |
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| 摘 要： | 目的：从甘草中提取分离α-葡萄糖苷酶抑制剂。方法：将甘草水提取物依次用石油醚、乙酸乙酯、正丁醇萃取，然后测定各部分的酶抑制活性，对活性最强的部分进行酶抑制动力学研究。结果：石油醚部分、乙酸乙酯部分、正丁醇部分与剩余水提物部分的酶活性抑制率分别为68．93％、83．02％、32．17％和10．79％，与综合水提取物的酶活性抑制率69．77％相比，乙酸乙酯部分的活性最强，而且乙酸乙酯部分表现为一种快速的剂量依赖性的竞争性抑制类型，其Ki=34μg／ml。结论：乙酸乙酯部分存在较强的α-葡萄糖苷酶抑制活性成分，对其作进一步分离可望得到α-葡萄糖苷酶抑制单体成分。
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| 关 键 词： | α-葡萄糖苷酶抑制剂 甘草 步研究 酶抑制活性 乙酸乙酯 正丁醇萃取 提取分离 水提取物 动力学研究 竞争性抑制 |
| 收稿时间： | 2005-05-24 |
| 修稿时间： | 2005年5月24日 |
Preliminary Study on the ol-glucosidase Inhibitor from Glycyrrhiza uralensis Fisch. |
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| Xu YouRui;Ni JingMan;Meng QingGang;Gao Yan;Fan Bin.Preliminary Study on the ol-glucosidase Inhibitor from Glycyrrhiza uralensis Fisch.[J].Jorunal of Chinese Medicinal Materials,2005,28(10):890-891. |
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| Authors: | Xu YouRui;Ni JingMan;Meng QingGang;Gao Yan;Fan Bin |
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| Institution: | School of Pharmacy, Lanzhou University, Lanzhou 730000. |
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| Abstract: | OBJECTIVE: To extract and isolate alpha-glucosidase inhibitor from Glycyrrhiza uralensis Fisch. METHODS: The aqueous extract of Glycyrrhiza uralensis Fisch. was extracted by petroleum, ethyl acetate and n-butanol. In addition, the inhibitory activity against alpha-glucosidase of every fraction was determined and the inhibitory kinetics of ethyl acetate fraction was investigated. RESULTS: The inhibitory activities of four fractions were 68.93%, 83.2%, 32.17% and 10.79% respectively. Compared with the aqueous extract (69.77%), the inhibitory activity of ethyl acetate fraction was higher. Ethyl acetate fraction was shown to be a fast-binding, dose-dependent and competitive model with a Ki value of 34 microg/ml. CONCLUSION: It is hoped to obtain good alpha-glucosidase inhibitors by separating ethyl acetate fraction of aqueous extract of Glycyrrhiza uralensis Fisch further. |
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